ArticleApplied and environmental microbiology2015
The Putative mevalonate diphosphate decarboxylase from Picrophilus torridus is in reality a mevalonate-3-kinase with high potential for bioproduction of isobutene.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Metabolome and transcriptome analyses reveal metabolic differences and identify candidate regulatory factors among three habitat types ofFrontiers in plant science · 2026Article
- Structure-guided engineering of α-ketoisocaproate dioxygenase increases isobutene production in Synechocystis sp. PCC 6803.Microbial cell factories · 2025Article
- Recent Advances and New Insights in Genome Analysis and Transcriptomic Approaches to Reveal Enzymes Associated with the Biosynthesis of Dendrobine-Type Sesquiterpenoid Alkaloids (DTSAs) from the Last Decade.Molecules (Basel, Switzerland) · 2024Review
- Article
- A [4Fe-4S] cluster resides at the active center of phosphomevalonate dehydratase, a key enzyme in the archaeal modified mevalonate pathway.Frontiers in microbiology · 2023Article
- Article
- Novel Homologs of Isopentenyl Phosphate Kinase Reveal Class-Wide Substrate Flexibility.ChemCatChem · 2021Article
- Directed evolution of prenylated FMN-dependent Fdc supports efficient in vivo isobutene production.Nature communications · 2021Article
- Isobutene production inMetabolic engineering communications · 2021Article
- Article
- Conversion of Mevalonate 3-Kinase into 5-Phosphomevalonate 3-Kinase by Single Amino Acid Mutations.Applied and environmental microbiology · 2019Article
- A linear pathway for mevalonate production supports growth of Thermococcus kodakarensis.Extremophiles : life under extreme conditions · 2019Article
- Metabolic engineering of Methylobacterium extorquens AM1 for the production of butadiene precursor.Microbial cell factories · 2018Article
- Modified mevalonate pathway of the archaeonProceedings of the National Academy of Sciences of the United States of America · 2018Article
- On the Origin of Isoprenoid Biosynthesis.Molecular biology and evolution · 2018Article
- Bio-production of gaseous alkenes: ethylene, isoprene, isobutene.Biotechnology for biofuels · 2018Review
- Characterization of feedback-resistant mevalonate kinases from the methanogenic archaeons Methanosaeta concilii and Methanocella paludicola.Microbiology (Reading, England) · 2017Article
- Lipid sugar carriers at the extremes: The phosphodolichols Archaea use in N-glycosylation.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Review
- Transcriptome Analysis of Genes Involved in Dendrobine Biosynthesis in Dendrobium nobile Lindl. Infected with Mycorrhizal Fungus MF23 (Mycena sp.).Scientific reports · 2017Article
- Mutation Analysis of the MVD Gene in a Chinese Family with Disseminated Superficial Actinic Porokeratosis and a Chinese Literature Review.Indian journal of dermatologyArticle
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Mevalonate diphosphate decarboxylase (MVD) is an ATP-dependent enzyme that catalyzes the phosphorylation/decarboxylation of (R)-mevalonate-5-diphosphate to isopentenyl pyrophosphate in the mevalonate (MVA) pathway. MVD is a key enzyme in engineered metabolic pathways for bioproduction of isobutene, since it catalyzes the conversion of 3-hydroxyisovalerate (3-HIV) to isobutene, an important platform chemical. The putative homologue from Picrophilus torridus has been identified as a highly efficient variant in a number of patents, but its detailed characterization has not been reported. In this study, we have successfully purified and characterized the putative MVD from P. torridus. We discovered that it is not a decarboxylase per se but an ATP-dependent enzyme, mevalonate-3-kinase (M3K), which catalyzes the phosphorylation of MVA to mevalonate-3-phosphate. The enzyme's potential in isobutene formation is due to the conversion of 3-HIV to an unstable 3-phosphate intermediate that undergoes consequent spontaneous decarboxylation to form isobutene. Isobutene production rates were as high as 507 pmol min(-1) g cells(-1) using Escherichia coli cells expressing the enzyme and 2,880 pmol min(-1) mg protein(-1) with the purified histidine-tagged enzyme, significantly higher than reported previously. M3K is a key enzyme of the novel MVA pathway discovered very recently in Thermoplasma acidophilum. We suggest that P. torridus metabolizes MVA by the same pathway.
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