ArticleApplied and environmental microbiology2015
Molecular gene profiling of Clostridium botulinum group III and its detection in naturally contaminated samples originating from various European countries.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Overview of botulinum neurotoxin-producing clostridia in soils in France.Microbiology spectrum · 2025Article
- Extensive Genome Exploration ofMicroorganisms · 2021Article
- Rapid Detection ofInternational journal of environmental research and public health · 2021Article
- Article
- Review
- Evaluation of the occurrence of sporulating and nonsporulating pathogenic bacteria in manure and in digestate of five agricultural biogas plants.MicrobiologyOpen · 2019Article
- New Insights into the Genetic Diversity of Clostridium botulinum Group III through Extensive Genome Exploration.Frontiers in microbiology · 2016Article
- Article
- Draft Genome Sequences of 17 French Clostridium botulinum Group III Strains.Genome announcements · 2015Article
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Authors and funding
15 authors.
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No grant is acknowledged in the PubMed record.
Abstract
We report the development of real-time PCR assays for genotyping Clostridium botulinum group III targeting the newly defined C. novyi sensu lato group; the nontoxic nonhemagglutinin (NTNH)-encoding gene ntnh; the botulinum neurotoxin (BoNT)-encoding genes bont/C, bont/C/D, bont/D, and bont/D/C; and the flagellin (fliC) gene. The genetic diversity of fliC among C. botulinum group III strains resulted in the definition of five major subgroups named fliC-I to fliC-V. Investigation of fliC subtypes in 560 samples, with various European origins, showed that fliC-I was predominant and found exclusively in samples contaminated by C. botulinum type C/D, fliC-II was rarely detected, no sample was recorded as fliC-III or fliC-V, and only C. botulinum type D/C samples tested positive for fliC-IV. The lack of genetic diversity of the flagellin gene of C. botulinum type C/D would support a clonal spread of type C/D strains in different geographical areas. fliC-I to fliC-III are genetically related (87% to 92% sequence identity), whereas fliC-IV from C. botulinum type D/C is more genetically distant from the other fliC types (with only 50% sequence identity). These findings suggest fliC-I to fliC-III have evolved in a common environment and support a different genetic evolution for fliC-IV. A combination of the C. novyi sensu lato, ntnh, bont, and fliC PCR assays developed in this study allowed better characterization of C. botulinum group III and showed the group to be less genetically diverse than C. botulinum groups I and II, supporting a slow genetic evolution of the strains belonging to C. botulinum group III.
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