ArticleMolecular genetics and genomics : MGG2015
Interactions of release factor RF3 with the translation machinery.
Article in Molecular genetics and genomics : MGG, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Suppressor analysis links trans-translation and ribosomal protein uS7 to RluD function in Escherichia coli.Biochemical and biophysical research communications · 2024Article
- Dynamics of release factor recycling during translation termination in bacteria.Nucleic acids research · 2023Article
- Alterations in the ribosomal protein bL12 of E. coli affecting the initiation, elongation and termination of protein synthesis.Biochimie · 2020Article
- Article
- Single-Molecule Fluorescence Applied to Translation.Cold Spring Harbor perspectives in biology · 2019Review
- Pseudouridine-Free Escherichia coli Ribosomes.Journal of bacteriology · 2018Article
- Post-termination Ribosome Intermediate Acts as the Gateway to Ribosome Recycling.Cell reports · 2017Article
- The C-terminus of ribosomal protein uS4 contributes to small ribosomal subunit biogenesis and the fidelity of translation.Biochimie · 2017Article
- Dynamic basis of fidelity and speed in translation: Coordinated multistep mechanisms of elongation and termination.Protein science : a publication of the Protein Society · 2017Review
- Global analysis of translation termination in E. coli.PLoS genetics · 2017Article
- The Loop 2 Region of Ribosomal Protein uS5 Influences Spectinomycin Sensitivity, Translational Fidelity, and Ribosome Biogenesis.Antimicrobial agents and chemotherapy · 2017Article
- Ribosomal frameshifting and transcriptional slippage: From genetic steganography and cryptography to adventitious use.Nucleic acids research · 2016Review
- Ribosome Structure Reveals Preservation of Active Sites in the Presence of a P-Site Wobble Mismatch.Structure (London, England : 1993) · 2015Article
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Abstract
The bacterial release factor RF3 is a GTPase that has been implicated in multiple, incompletely understood steps of protein synthesis. This study explores the genetic interaction of RF3 with other components of the translation machinery. RF3 contributes to translation termination by recycling the class I release factors RF1 and RF2 off post-termination ribosomes. RF3 has also been implicated in dissociation of peptidyl-tRNAs from elongating ribosomes and in a post-peptidyltransferase quality control (post-PT QC) mechanism that selectively terminates ribosomes carrying erroneous peptides. A majority of the in vivo studies on RF3 have been carried out in K-12 strains of Escherichia coli which carry a partially defective RF2 protein with an Ala to Thr substitution at position 246. Here, the contribution of the K-12 specific RF2 variant to RF3 activities has been investigated. Strain reconstruction experiments in both E. coli and Salmonella enterica demonstrate that defects in termination and post-PT QC that are associated with RF3 loss, as well as phenotypes uncovered by phenotypic profiling, are all substantially ameliorated when the incompletely active K-12-specific RF2 protein is replaced by a fully active Ala246 RF2. These results indicate that RF3 loss is well tolerated in bacteria with fully active class I release factors, but that many of the previously reported phenotypes for RF3 deletion strains have been compromised by the presence of a partially defective RF2.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.