ArticleMolecular endocrinology (Baltimore, Md.)2015
Rapid communication: A microRNA-132/212 pathway mediates GnRH activation of FSH expression.
Article in Molecular endocrinology (Baltimore, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 59 citations in OpenAlex.
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- Insights into pubertal development: a narrative review on the role of epigenetics.Journal of endocrinological investigation · 2025Review
- Gene-environment interaction in functional hypothalamic amenorrhea.Frontiers in endocrinology · 2024Review
- Peptidylarginine deiminase 2 regulates expression of DGCR8 affecting miRNA biogenesis in gonadotrope cells.Reproduction (Cambridge, England) · 2023Article
- Impaired BCAA catabolism in adipose tissues promotes age-associated metabolic derangement.Nature aging · 2023Article
- SIRT1: A Key Player in Male Reproduction.Life (Basel, Switzerland) · 2022Review
- FOXO Transcription Factors Are Required for Normal Somatotrope Function and Growth.Endocrinology · 2022Article
- Editorial: MicroRNAs in endocrinology and cell signaling.Frontiers in endocrinology · 2022Article
- Epigenetics of functional hypothalamic amenorrhea.Frontiers in endocrinology · 2022Review
- Advances in the Regulation of Mammalian Follicle-Stimulating Hormone Secretion.Animals : an open access journal from MDPI · 2021Review
- Post-Transcriptional Regulation ofInternational journal of molecular sciences · 2021Review
- Sirt1 Activity in the Brain: Simultaneous Effects on Energy Homeostasis and Reproduction.International journal of environmental research and public health · 2021Review
- Association of microRNAs With Embryo Development and Fertilization in Women Undergoing Subfertility Treatments: A Pilot Study.Frontiers in reproductive health · 2021Article
- Identification and validation of key miRNAs and miRNA-mRNA regulatory network associated with uterine involution in postpartum Kazakh sheep.Archives animal breeding · 2021Article
- An Exploration of Non-Coding RNAs in Extracellular Vesicles Delivered by Swine Anterior Pituitary.Frontiers in genetics · 2021Article
- Differential expression profiles of microRNAs in musk gland of unmated and mated forest musk deer (PeerJ · 2021Article
- MiR-130a-3p Inhibits PRL Expression and Is Associated With Heat Stress-Induced PRL Reduction.Frontiers in endocrinology · 2020Article
- The Versatility of Sirtuin-1 in Endocrinology and Immunology.Frontiers in cell and developmental biology · 2020Review
- circ-Sirt1 controls NF-κB activation via sequence-specific interaction and enhancement of SIRT1 expression by binding to miR-132/212 in vascular smooth muscle cells.Nucleic acids research · 2019Article
- Neuronal SIRT1 Regulates Metabolic and Reproductive Function and the Response to Caloric Restriction.Journal of the Endocrine Society · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
GnRH plays a key role in the vertebrate reproductive system by stimulating biosynthesis and secretion of pituitary gonadotropins. However, the potential involvement of microRNAs (miRNAs) on this activation has still to be explored. In this study, we investigated the role of miRNA-132 and miRNA-212, two tandemly expressed miRNAs that target the same transcripts, on GnRH-induced FSH expression. We first showed that the GnRH stimulation of FSH secretion was reduced and Fshb mRNA abolished by blocking miR-132/212 action in rat pituitary cells. In mouse LβT2 gonadotrope cells, the GnRH stimulation of Fshb mRNA was also demonstrated to be dependent on miR-132/212 and reproduced by overexpressing one or both miRNAs. We then showed that the miR-132/212-mediated action of GnRH involved a posttranscriptional decrease of sirtuin 1 (SIRT1) deacetylase. The lower level of SIRT1 deacetylase correlated with an increase in the acetylated form of Forkhead Box O1 (FOXO1), a transcriptional repressor of Fshb. Interestingly, we show that the acetylated mimicking mutant of FOXO1 was localized outside the nucleus, thus alleviating its repressive effect on Fshb transcription. Overall, we demonstrate that the GnRH stimulation of Fshb expression is dependent on miR-132/212 and involves a SIRT1-FOXO1 pathway. This is the first demonstration of an obligatory microRNA pathway in the GnRH-regulated expression of a gonadotropin gene.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.