ArticleNature communications2015
Complete architecture of the archaeal RNA polymerase open complex from single-molecule FRET and NPS.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 67 citations in OpenAlex.
- Dynamic positioning of Rpc34 winged helix in RNA polymerase III elongation complex for its stability with implications for reinitiation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Promoter contacts by TFB and TFEα during archaeal transcription initiation.Transcription · 2026Article
- Determination of absolute intramolecular distances in proteins using anomalous X-ray scattering interferometry.Nanoscale · 2025Article
- Structural basis of archaeal RNA polymerase transcription elongation and Spt4/5 recruitment.Nucleic acids research · 2024Article
- Promoter-proximal elongation regulates transcription in archaea.Nature communications · 2021Article
- Multimodal single-molecule microscopy with continuously controlled spectral resolution.Biophysical reports · 2021Article
- Integrating single-molecule FRET and biomolecular simulations to study diverse interactions between nucleic acids and proteins.Essays in biochemistry · 2021Review
- FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices.eLife · 2021Review
- Elucidation of the Translation Initiation Factor Interaction Network ofFrontiers in microbiology · 2021Article
- The smfBox is an open-source platform for single-molecule FRET.Nature communications · 2020Article
- Archaeal transcription.Transcription · 2020Review
- Stepwise Promoter Melting by Bacterial RNA Polymerase.Molecular cell · 2020Article
- FttA is a CPSF73 homologue that terminates transcription in Archaea.Nature microbiology · 2020Article
- The cutting edge of archaeal transcription.Emerging topics in life sciences · 2018Article
- Displacement of the transcription factor B reader domain during transcription initiation.Nucleic acids research · 2018Article
- A transcriptional factor B paralog functions as an activator to DNA damage-responsive expression in archaea.Nucleic acids research · 2018Article
- Caging and Photoactivation in Single-Molecule Förster Resonance Energy Transfer Experiments.Biochemistry · 2017Article
- A global analysis of transcription reveals two modes of Spt4/5 recruitment to archaeal RNA polymerase.Nature microbiology · 2017Article
- Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System.Journal of visualized experiments : JoVE · 2017Article
- Cool and dynamic: single-molecule fluorescence-based structural biology.Nature methods · 2017Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The molecular architecture of RNAP II-like transcription initiation complexes remains opaque due to its conformational flexibility and size. Here we report the three-dimensional architecture of the complete open complex (OC) composed of the promoter DNA, TATA box-binding protein (TBP), transcription factor B (TFB), transcription factor E (TFE) and the 12-subunit RNA polymerase (RNAP) from Methanocaldococcus jannaschii. By combining single-molecule Förster resonance energy transfer and the Bayesian parameter estimation-based Nano-Positioning System analysis, we model the entire archaeal OC, which elucidates the path of the non-template DNA (ntDNA) strand and interaction sites of the transcription factors with the RNAP. Compared with models of the eukaryotic OC, the TATA DNA region with TBP and TFB is positioned closer to the surface of the RNAP, likely providing the mechanism by which DNA melting can occur in a minimal factor configuration, without the dedicated translocase/helicase encoding factor TFIIH.
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