ArticlePloS one2015
Comparison of eleven methods for genomic DNA extraction suitable for large-scale whole-genome genotyping and long-term DNA banking using blood samples.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed, 171 citations in OpenAlex.
- Comparison of Whole Exome Sequencing Commercial Kits Performance Across Diverse Tissue Sources.International journal of molecular sciences · 2026Article
- A Green and Practical Magnetic Ionic Liquid-Based Microextraction of DNA Using a Low-Cost 3D-Printed Open-Source Apparatus.ACS omega · 2026Article
- A rapid and high-yield method for nucleic acid extraction.Scientific reports · 2025Article
- Characteristics ofJournal of fungi (Basel, Switzerland) · 2025Article
- Microfluidic purification of genomic DNA.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Molecular and Functional Analysis of the Stearoyl-CoA Desaturase (SCD) Gene in Buffalo: Implications for Milk Fat Synthesis.Animals : an open access journal from MDPI · 2024Article
- Peripheral blood to next-generation sequencing ready DNA library: a novel engineering design for automation.BMC genomics · 2024Article
- Phylogenetic analysis ofOpen veterinary journal · 2024Article
- A DNA Extraction Method for Nondestructive Testing and Evaluation of Cotton Seeds (Gossypium L.).Biochemical genetics · 2024Article
- A rapid and inexpensive 96-well DNA-extraction method from blood using silicon dioxide powder (Glassmilk).Biology methods & protocols · 2024Article
- DNA Isolation Long-Read Genomic Sequencing in Ctenophores.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Systems Biology and Inborn Error of Metabolism: Analytical Strategy in Investigating Different Biochemical/Genetic Parameters.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Comparison of 3 DNA extraction methods for extracting DNA from an adult Culex quinquefasciatus (Diptera: Culicidae).Journal of insect science (Online) · 2023Article
- An evaluation of DNA extraction methods on historical and roadkill mammalian specimen.Scientific reports · 2023Article
- Article
- An efficient, fast and inexpensive method for genomic DNA extraction of fish tissue.Molecular biology reports · 2023Article
- Sensitive and Quantitative Point-of-Care HIV Viral Load Quantification from Blood Using a Power-Free Plasma Separation and Portable Magnetofluidic Polymerase Chain Reaction Instrument.Analytical chemistry · 2023Article
- A Guide to Genome-Wide Association Study Design for Diabetic Retinopathy.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Over the recent years, next generation sequencing and microarray technologies have revolutionized scientific research with their applications to high-throughput analysis of biological systems. Isolation of high quantities of pure, intact, double stranded, highly concentrated, not contaminated genomic DNA is prerequisite for successful and reliable large scale genotyping analysis. High quantities of pure DNA are also required for the creation of DNA-banks. In the present study, eleven different DNA extraction procedures, including phenol-chloroform, silica and magnetic beads based extractions, were examined to ascertain their relative effectiveness for extracting DNA from ovine blood samples. The quality and quantity of the differentially extracted DNA was subsequently assessed by spectrophotometric measurements, Qubit measurements, real-time PCR amplifications and gel electrophoresis. Processing time, intensity of labor and cost for each method were also evaluated. Results revealed significant differences among the eleven procedures and only four of the methods yielded satisfactory outputs. These four methods, comprising three modified silica based commercial kits (Modified Blood, Modified Tissue, Modified Dx kits) and an in-house developed magnetic beads based protocol, were most appropriate for extracting high quality and quantity DNA suitable for large-scale microarray genotyping and also for long-term DNA storage as demonstrated by their successful application to 600 individuals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.