ArticleThe Journal of biological chemistry2015
Evolutionarily conserved binding of translationally controlled tumor protein to eukaryotic elongation factor 1B.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- The ER anchoring and abundance of the EEF1B complex is affected by tissue-specific alternative EEF1D splicing.Life science alliance · 2026Article
- Quaternary organization of the human eEF1B complex reveals unique multi-GEF domain assembly.Nucleic acids research · 2022Article
- Prediction of amphipathic helix-membrane interactions with Rosetta.PLoS computational biology · 2021Article
- Discovery and mechanism of a pH-dependent dual-binding-site switch in the interaction of a pair of protein modules.Science advances · 2020Article
- Review
- Article
- Systemic signalling through translationally controlled tumour protein controls lateral root formation in Arabidopsis.Journal of experimental botany · 2019Article
- Alternative σI/anti-σI factors represent a unique form of bacterial σ/anti-σ complex.Nucleic acids research · 2019Article
- Identification of a Specific Translational Machinery via TCTP-EF1A2 Interaction Regulating NF1-associated Tumor Growth by Affinity Purification and Data-independent Mass Spectrometry Acquisition (AP-DIA).Molecular & cellular proteomics : MCP · 2019Article
- Some Biological Consequences of the Inhibition of Na,K-ATPase by Translationally Controlled Tumor Protein (TCTP).International journal of molecular sciences · 2018Review
- Insights into the role and regulation of TCTP in skeletal muscle.Oncotarget · 2017Article
- Translationally controlled tumour protein TCTP is induced early in human colorectal tumours and contributes to the resistance of HCT116 colon cancer cells to 5-FU and oxaliplatin.Cell communication and signaling : CCS · 2017Article
- The C-terminal region of human eukaryotic elongation factor 1Bδ.Journal of biomolecular NMR · 2016Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Translationally controlled tumor protein (TCTP) is an abundant protein that is highly conserved in eukaryotes. However, its primary function is still not clear. Human TCTP interacts with the metazoan-specific eukaryotic elongation factor 1Bδ (eEF1Bδ) and inhibits its guanine nucleotide exchange factor (GEF) activity, but the structural mechanism remains unknown. The interaction between TCTP and eEF1Bδ was investigated by NMR titration, structure determination, paramagnetic relaxation enhancement, site-directed mutagenesis, isothermal titration calorimetry, and HADDOCK docking. We first demonstrated that the catalytic GEF domain of eEF1Bδ is not responsible for binding to TCTP but rather a previously unnoticed central acidic region (CAR) domain in eEF1Bδ. The mutagenesis data and the structural model of the TCTP-eEF1Bδ CAR domain complex revealed the key binding residues. These residues are highly conserved in eukaryotic TCTPs and in eEF1B GEFs, including the eukaryotically conserved eEF1Bα, implying the interaction may be conserved in all eukaryotes. Interactions were confirmed between TCTP and the eEF1Bα CAR domain for human, fission yeast, and unicellular photosynthetic microalgal proteins, suggesting that involvement in protein translation through the conserved interaction with eEF1B represents a primary function of TCTP.
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