ArticleNucleic acids research2015
Sequence-specific cleavage of dsRNA by Mini-III RNase.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Systems-level analysis predicts no autotrophy-linked protein-RNA interactions in Clostridium autoethanogenum.Nature communications · 2026Article
- Structural and functional investigation of the DHH/DHHA1 family proteins in Deinococcus radiodurans.Nucleic acids research · 2024Article
- Review
- Proteomic andFrontiers in microbiology · 2022Article
- Mini-III RNase-based dual-color system forDevelopment (Cambridge, England) · 2020Article
- Structures of B. subtilis Maturation RNases Captured on 50S Ribosome with Pre-rRNAs.Molecular cell · 2020Article
- Article
- Bacterial ribonucleases and their roles in RNA metabolism.Critical reviews in biochemistry and molecular biology · 2019Review
- The molecular mechanism of dsRNA processing by a bacterial Dicer.Nucleic acids research · 2019Article
- A Minigenome Study of Hazara Nairovirus Genomic Promoters.Journal of virology · 2019Article
- In vivo cleavage rules and target repertoire of RNase III in Escherichia coli.Nucleic acids research · 2018Article
- Review
- Article
- TheFrontiers in physiology · 2018Article
- Identification of protein structural elements responsible for the diversity of sequence preferences among Mini-III RNases.Scientific reports · 2016Article
- Nucleotide sequence of miRNA precursor contributes to cleavage site selection by Dicer.Nucleic acids research · 2015Article
- Viral Small-RNA Analysis of Bombyx mori Larval Midgut during Persistent and Pathogenic Cytoplasmic Polyhedrosis Virus Infection.Journal of virology · 2015Article
- Sequence features of Drosha and Dicer cleavage sites affect the complexity of isomiRs.International journal of molecular sciences · 2015Article
- Local Mutational Pressures in Genomes of Zaire Ebolavirus and Marburg Virus.Advances in bioinformatics · 2015Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ribonucleases (RNases) play a critical role in RNA processing and degradation by hydrolyzing phosphodiester bonds (exo- or endonucleolytically). Many RNases that cut RNA internally exhibit substrate specificity, but their target sites are usually limited to one or a few specific nucleotides in single-stranded RNA and often in a context of a particular three-dimensional structure of the substrate. Thus far, no RNase counterparts of restriction enzymes have been identified which could cleave double-stranded RNA (dsRNA) in a sequence-specific manner. Here, we present evidence for a sequence-dependent cleavage of long dsRNA by RNase Mini-III from Bacillus subtilis (BsMiniIII). Analysis of the sites cleaved by this enzyme in limited digest of bacteriophage Φ6 dsRNA led to the identification of a consensus target sequence. We defined nucleotide residues within the preferred cleavage site that affected the efficiency of the cleavage and were essential for the discrimination of cleavable versus non-cleavable dsRNA sequences. We have also determined that the loop α5b-α6, a distinctive structural element in Mini-III RNases, is crucial for the specific cleavage, but not for dsRNA binding. Our results suggest that BsMiniIII may serve as a prototype of a sequence-specific dsRNase that could possibly be used for targeted cleavage of dsRNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.