ArticlePLoS genetics2015
MiR-24 is required for hematopoietic differentiation of mouse embryonic stem cells.
Article in PLoS genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Extracellular Vesicles from Canine Mesenchymal Stem Cells-Isolation, Characterization and miRNA Definition Following Interleukin-1ß and Shockwave Treatment.Animals : an open access journal from MDPI · 2026Article
- Human erythrocytes' perplexing behaviour: erythrocytic microRNAs.Molecular and cellular biochemistry · 2025Review
- MicroRNA target homeobox messenger RNA in HIV induced hematopoietic inhibition.Frontiers in cell and developmental biology · 2024Article
- [Tribbles pseudokinase 3 inhibits the adipogenic differentiation of human adipose-derived mesenchymal stem cells].Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2020Article
- miRNA-24 Gene Sequence, DHFR -829C-T Genotypes, and Methotrexate Response in Mexican Patients with Rheumatoid Arthritis.Genetic testing and molecular biomarkers · 2019Article
- The mirn23a and mirn23b microrna clusters are necessary for proper hematopoietic progenitor cell production and differentiation.Experimental hematology · 2018Article
- Evidence for a role of TRIB3 in the regulation of megakaryocytopoiesis.Scientific reports · 2017Article
- The miR-23a~27a~24-2 microRNA cluster buffers transcription and signaling pathways during hematopoiesis.PLoS genetics · 2017Article
- Article
- Low-cell-number, single-tube amplification (STA) of total RNA revealed transcriptome changes from pluripotency to endothelium.BMC biology · 2017Article
- Human Embryos Created by Embryo Splitting Secrete Significantly Lower Levels of miRNA-30c.Stem cells and development · 2016Article
- Progress towards generation of human haematopoietic stem cells.Nature cell biology · 2016Review
- Inhibition of human primary megakaryocyte differentiation by anagrelide: a gene expression profiling analysis.International journal of hematology · 2016Article
- Molecular Analysis of Neutrophil Differentiation from Human Induced Pluripotent Stem Cells Delineates the Kinetics of Key Regulators of Hematopoiesis.Stem cells (Dayton, Ohio) · 2016Article
- Evaluation of the mirn23a Cluster through an iTRAQ-based Quantitative Proteomic Approach.Journal of proteome research · 2016Article
- MicroRNAs (miRNAs) in the control of HF development and cycling: the next frontiers in hair research.Experimental dermatology · 2015Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Overexpression of miRNA, miR-24, in mouse hematopoietic progenitors increases monocytic/ granulocytic differentiation and inhibits B cell development. To determine if endogenous miR-24 is required for hematopoiesis, we antagonized miR-24 in mouse embryonic stem cells (ESCs) and performed in vitro differentiations. Suppression of miR-24 resulted in an inability to produce blood and hematopoietic progenitors (HPCs) from ESCs. The phenotype is not a general defect in mesoderm production since we observe production of nascent mesoderm as well as mesoderm derived cardiac muscle and endothelial cells. Results from blast colony forming cell (BL-CFC) assays demonstrate that miR-24 is not required for generation of the hemangioblast, the mesoderm progenitor that gives rise to blood and endothelial cells. However, expression of the transcription factors Runx1 and Scl is greatly reduced, suggesting an impaired ability of the hemangioblast to differentiate. Lastly, we observed that known miR-24 target, Trib3, is upregulated in the miR-24 antagonized embryoid bodies (EBs). Overexpression of Trib3 alone in ESCs was able to decrease HPC production, though not as great as seen with miR-24 knockdown. These results demonstrate an essential role for miR-24 in the hematopoietic differentiation of ESCs. Although many miRNAs have been implicated in regulation of hematopoiesis, this is the first miRNA observed to be required for the specification of mammalian blood progenitors from early mesoderm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.