Evidence map›Paper›PMID 25633484›Full record

ArticleCancer gene therapy2015

MMP-9 is increased in the pathogenesis of gastric cancer by the mediation of HER2.

Y-Q Shan, R-C Ying, C-H Zhou, A-K Zhu, J Ye, W Zhu, T-F Ju, H-C Jin

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Cancer gene therapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
3.2field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 45 citations in OpenAlex.

  1. Review
  2. Introducing anxiety as a risk factor of gastric cancer.Gastroenterology and hepatology from bed to bench · 2026
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  9. Quercetin exerts anti-inflammatory effectsWorld journal of gastroenterology · 2022
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  17. Article
  18. Article
  19. THE ROLE OF SERUM CK-18, MMP-9 AND TIPM-1 LEVELS IN PREDICTING R0 RESECTION IN PATIENTS WITH GASTRIC CANCER.Arquivos brasileiros de cirurgia digestiva : ABCD = Brazilian archives of digestive surgery · 2018
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Y-Q Shan1] Department of General Surgery, Hangzhou First People's Hospital, Hangzhou, China [2] Affiliated Hangzhou Hospital, Nanjing Medical University, Hangzhou, China.
R-C Ying1] Department of General Surgery, Hangzhou First People's Hospital, Hangzhou, China [2] Affiliated Hangzhou Hospital, Nanjing Medical University, Hangzhou, China.
C-H Zhou1] Department of General Surgery, Hangzhou First People's Hospital, Hangzhou, China [2] Affiliated Hangzhou Hospital, Nanjing Medical University, Hangzhou, China.
A-K Zhu1] Department of General Surgery, Hangzhou First People's Hospital, Hangzhou, China [2] Affiliated Hangzhou Hospital, Nanjing Medical University, Hangzhou, China.
J Ye1] Department of General Surgery, Hangzhou First People's Hospital, Hangzhou, China [2] Affiliated Hangzhou Hospital, Nanjing Medical University, Hangzhou, China.
W Zhu1] Department of General Surgery, Hangzhou First People's Hospital, Hangzhou, China [2] Affiliated Hangzhou Hospital, Nanjing Medical University, Hangzhou, China.
T-F Ju1] Department of General Surgery, Hangzhou First People's Hospital, Hangzhou, China [2] Affiliated Hangzhou Hospital, Nanjing Medical University, Hangzhou, China.
H-C Jin1] Department of General Surgery, Hangzhou First People's Hospital, Hangzhou, China [2] Affiliated Hangzhou Hospital, Nanjing Medical University, Hangzhou, China.
Hangzhou First People's Hospital · CNNanjing Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human epidermal growth factor receptor 2 (HER2) overexpression is not only closely associated with the tumor growth, but is also related to tumor invasion. We here aimed to investigate the mechanism of HER2 mediation in the pathogenesis of gastric cancer. The human gastric cancer cell lines SGC-7901, MKN-45, AGS, the immortalized cell line GES-1 derived from normal gastric mucosa. Cell transfection and selection of stable cell lines and the gene and protein levels of HER2 and Matrix metalloproteinase-9 (MMP-9) were examined to determine the molecular relationship between them in the pathogenesis of gastric cancer. The human gastric cancer cell lines SGC-7901, MKN-45, AGS, the immortalized cell line GES-1 derived from normal gastric mucosa. Cell transfection and selection of stable cell lines and the gene and protein levels of HER2 and MMP-9 were examined to determine the molecular relationship between them in the pathogenesis of gastric cancer. We demonstrated that vector-based shRNA significantly knocked down the expression of HER2 and considerably inhibited both the migration and invasion of gastric cancer cells. HER2 knockdown resulted in the downregulation of the expression of MMP-9, whereas HER2 overexpression improved the transcription of MMP-9 through the activation of an MMP-9 promoter. The promoter region of MMP-9 between -2500 and -2000 bp was found to be crucial for the upregulation of HER2-mediated transcription. Furthermore, a truncated promoter (-70 to +63) did not display any transcriptional activity. Cell invasion activity was almost completely inhibited when MMP-9 was knocked down. Conversely, the overexpression of MMP-9 partly rescued the invasion ability of cell strains with knockdown HER2. These findings help further understanding of the molecular mechanisms through which HER2 promotes malignancy, and suggest that targeting both HER2 and MMP-9 may be required to effectively block HER2 signaling in gastric cancer therapy.

Indexed as

Cell Line, TumorCell MovementEnzyme InductionErb-b2 Receptor Tyrosine KinasesGene ExpressionGene Expression Regulation, NeoplasticGene Knockdown TechniquesHumansMatrix Metalloproteinase 9Neoplasm InvasivenessPromoter Regions, GeneticRNA, Small InterferingStomach NeoplasmsTranscriptional ActivationERBB2 protein, humanErb-b2 Receptor Tyrosine KinasesMatrix Metalloproteinase 9MMP9 protein, humanRNA, Small Interfering

Identifiers

PMID25633484
OpenAlexW1976208151

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.