Evidence map›Paper›PMID 25633331›Full record

ArticleMolecules (Basel, Switzerland)2015

Development and validation of 697 novel polymorphic genomic and EST-SSR markers in the American cranberry (Vaccinium macrocarpon Ait.).

Brandon Schlautman, Diego Fajardo, Tierney Bougie, Eric Wiesman, James Polashock, Nicholi Vorsa, Shawn Steffan, Juan Zalapa

Abstract read
In one paragraph

Article in Molecules (Basel, Switzerland), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Genetic Diversity and Identification ofPlants (Basel, Switzerland) · 2024
    Article
  2. Intersectional Hybrids between Darrow's Blueberry (Plants (Basel, Switzerland) · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Brandon SchlautmanDepartment of Horticulture, University of Wisconsin-Madison, 1575 Linden Dr. Madison, WI 53706, USA. schlautman@wisc.edu.
Diego FajardoNational Center for Genome Resources, 2935 Rodeo Park Dr. East, Sante Fe, NM 87505, USA. dfajardo@ncgr.org.
Tierney BougieDepartment of Horticulture, University of Wisconsin-Madison, 1575 Linden Dr. Madison, WI 53706, USA. tcbougie@wisc.edu.
Eric WiesmanUSDA-ARS, Vegetable Crops Research Unit, University of Wisconsin, Madison, WI 53706, USA. eric.wiesman@ars.usda.gov.
James PolashockUSDA-ARS, Genetic Improvement of Fruits and Vegetables Laboratory, Rutgers University Chatsworth, NJ 08019, USA. james.polashock@ars.usda.gov.
Nicholi VorsaBlueberry and Cranberry Research and Extension Center, Rutgers University, Chatsworth, NJ 08019, USA. vorsa@aesop.rutgers.edu.
Shawn SteffanUSDA-ARS, Vegetable Crops Research Unit, University of Wisconsin, Madison, WI 53706, USA. shawn.steffan@ars.usda.gov.
Juan ZalapaDepartment of Horticulture, University of Wisconsin-Madison, 1575 Linden Dr. Madison, WI 53706, USA. jezalapa@wisc.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The American cranberry, Vaccinium macrocarpon Ait., is an economically important North American fruit crop that is consumed because of its unique flavor and potential health benefits. However, a lack of abundant, genome-wide molecular markers has limited the adoption of modern molecular assisted selection approaches in cranberry breeding programs. To increase the number of available markers in the species, this study identified, tested, and validated microsatellite markers from existing nuclear and transcriptome sequencing data. In total, new primers were designed, synthesized, and tested for 979 SSR loci; 697 of the markers amplified allele patterns consistent with single locus segregation in a diploid organism and were considered polymorphic. Of the 697 polymorphic loci, 507 were selected for additional genetic diversity and segregation analyses in 29 cranberry genotypes. More than 95% of the 507 loci did not display segregation distortion at the p < 0.05 level, and contained moderate to high levels of polymorphism with a polymorphic information content >0.25. This comprehensive collection of developed and validated microsatellite loci represents a substantial addition to the molecular tools available for geneticists, genomicists, and breeders in cranberry and Vaccinium.

Indexed as

Expressed Sequence TagsMicrosatellite RepeatsGenes, PlantGenetic MarkersPolymorphism, GeneticSequence Analysis, DNASpecies SpecificityVaccinium macrocarponGenetic Markers

Identifiers

PMID25633331
PMCPMC6272188

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.