ArticlePLoS pathogens2015
Elucidation of the RamA regulon in Klebsiella pneumoniae reveals a role in LPS regulation.
Article in PLoS pathogens, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 83 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
83 citing papers in PubMed, 160 citations in OpenAlex.
- Cyclic di-GMP directly reprograms the multidrug resistance machinery via UspG-mediated sequestration of RamR in Klebsiella.PLoS pathogens · 2026Article
- Structure-Function Correlations of Commercial Fucoidan Extracts: Antioxidant, Antiviral, Antifungal, Antibacterial and Prebiotic Activities.Molecules (Basel, Switzerland) · 2026Article
- A review on hypervirulent and multidrug-resistant Klebsiella pneumoniae: An emerging threat to human health.Naunyn-Schmiedeberg's archives of pharmacology · 2026Review
- Overexpression of Escherichia coli yaiX Confers Multidrug Resistance and Enhances Virulence in the Silkworm Infection Model.Microbiology and immunology · 2026Article
- Carbapenem resistance and hypervirulence iniScience · 2026Review
- A specific amount of RamA must be reached to trigger increased expression of AcrAB, enhance efflux, and confer multidrug resistance.Nucleic acids research · 2026Article
- Genomic characterization of multidrug-resistant Klebsiella pneumoniae from an outbreak in Northeastern Brazil: mechanisms of virulence and resistance.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Immune evasion of multidrug-resistant bacteria: insights from lung innate immune cells and targeted therapies.Frontiers in cellular and infection microbiology · 2026Review
- Strain-dependent contribution of the AcrAB-TolC efflux pump toMicrobiology (Reading, England) · 2025Article
- The resilience ofmSphere · 2025Article
- Insights into the Metabolic Adaptations of a Carbapenem-ResistantInternational journal of molecular sciences · 2025Article
- How Klebsiella pneumoniae controls its virulence.PLoS pathogens · 2025Review
- Antibiotic-induced stress responses in Gram-negative bacteria and their role in antibiotic resistance.The Journal of antimicrobial chemotherapy · 2025Review
- Coordination of cell envelope biology by Escherichia coli MarA protein potentiates intrinsic antibiotic resistance.PLoS genetics · 2025Article
- Rapid and Integrated Bacterial Evolution Analysis unveils gene mutations and clinical risk of Klebsiella pneumoniae.Nature communications · 2025Article
- Emergence of concurrently transmissibleMicrobiology spectrum · 2025Article
- RamA upregulates the ATP-binding cassette transporterMicrobiology spectrum · 2025Article
- Mechanisms of Antimicrobial Resistance inInfection and drug resistance · 2025Review
- Phenotypic and genomic changes in entericmSphere · 2024Article
- Understanding carbapenem-resistant hypervirulenthLife · 2024Review
23 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issuedCorrection: Elucidation of the RamA regulon in Klebsiella pneumoniae reveals a role in LPS regulation.2015 · Monaghan, Avril [corrected to Monahan, Avril]
- Erratum issued
Authors and funding
16 authors at 6 institutions in 2 countries.
Funding
Abstract
Klebsiella pneumoniae is a significant human pathogen, in part due to high rates of multidrug resistance. RamA is an intrinsic regulator in K. pneumoniae established to be important for the bacterial response to antimicrobial challenge; however, little is known about its possible wider regulatory role in this organism during infection. In this work, we demonstrate that RamA is a global transcriptional regulator that significantly perturbs the transcriptional landscape of K. pneumoniae, resulting in altered microbe-drug or microbe-host response. This is largely due to the direct regulation of 68 genes associated with a myriad of cellular functions. Importantly, RamA directly binds and activates the lpxC, lpxL-2 and lpxO genes associated with lipid A biosynthesis, thus resulting in modifications within the lipid A moiety of the lipopolysaccharide. RamA-mediated alterations decrease susceptibility to colistin E, polymyxin B and human cationic antimicrobial peptide LL-37. Increased RamA levels reduce K. pneumoniae adhesion and uptake into macrophages, which is supported by in vivo infection studies, that demonstrate increased systemic dissemination of ramA overexpressing K. pneumoniae. These data establish that RamA-mediated regulation directly perturbs microbial surface properties, including lipid A biosynthesis, which facilitate evasion from the innate host response. This highlights RamA as a global regulator that confers pathoadaptive phenotypes with implications for our understanding of the pathogenesis of Enterobacter, Salmonella and Citrobacter spp. that express orthologous RamA proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.