ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2015
An acto-myosin II constricting ring initiates the fission of activity-dependent bulk endosomes in neurosecretory cells.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- The epithelial NaCell reports · 2023Article
- Advances and challenges in understanding endosomal sorting and fission.The FEBS journal · 2023Article
- Sequential compound fusion and kiss-and-run mediate exo- and endocytosis in excitable cells.Science advances · 2022Article
- Minibrain kinase and calcineurin coordinate activity-dependent bulk endocytosis through synaptojanin.The Journal of cell biology · 2021Article
- Article
- Radial contractility of actomyosin rings facilitates axonal trafficking and structural stability.The Journal of cell biology · 2020Article
- Activity-dependent bulk endocytosis proteome reveals a key presynaptic role for the monomeric GTPase Rab11.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The ever-growing complexity of the mitochondrial fission machinery.Cellular and molecular life sciences : CMLS · 2018Review
- Endophilin A and B Join Forces With Clathrin to Mediate Synaptic Vesicle Recycling inFrontiers in molecular neuroscience · 2018Article
- Myosin II activity is required for structural plasticity at the axon initial segment.The European journal of neuroscience · 2017Article
- Integration of Synaptic Vesicle Cargo Retrieval with Endocytosis at Central Nerve Terminals.Frontiers in cellular neuroscience · 2017Review
- Dynamin-1 deletion enhances post-tetanic potentiation and quantal size after tetanic stimulation at the calyx of Held.The Journal of physiology · 2017Article
- Myosin light chain kinase facilitates endocytosis of synaptic vesicles at hippocampal boutons.Journal of neurochemistry · 2016Article
- Tissue-specific dynamin-1 deletion at the calyx of Held decreases short-term depression through a mechanism distinct from vesicle resupply.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Imaging membrane remodeling during regulated exocytosis in live mice.Experimental cell research · 2015Review
- Small molecules demonstrate the role of dynamin as a bi-directional regulator of the exocytosis fusion pore and vesicle release.Molecular psychiatry · 2015Article
- Myosin II and dynamin control actin rings to mediate fission during activity-dependent bulk endocytosis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Activity-dependent bulk endocytosis allows neurons to internalize large portions of the plasma membrane in response to stimulation. However, whether this critical type of compensatory endocytosis is unique to neurons or also occurs in other excitable cells is currently unknown. Here we used fluorescent 70 kDa dextran to demonstrate that secretagogue-induced bulk endocytosis also occurs in bovine chromaffin cells. The relatively large size of the bulk endosomes found in this model allowed us to investigate how the neck of the budding endosomes constricts to allow efficient recruitment of the fission machinery. Using time-lapse imaging of Lifeact-GFP-transfected chromaffin cells in combination with fluorescent 70 kDa dextran, we detected acto-myosin II rings surrounding dextran-positive budding endosomes. Importantly, these rings were transient and contracted before disappearing, suggesting that they might be involved in restricting the size of the budding endosome neck. Based on the complete recovery of dextran fluorescence after photobleaching, we demonstrated that the actin ring-associated budding endosomes were still connected with the extracellular fluid. In contrast, no such recovery was observed following the constriction and disappearance of the actin rings, suggesting that these structures were pinched-off endosomes. Finally, we showed that the rings were initiated by a circular array of phosphatidylinositol(4,5)bisphosphate microdomains, and that their constriction was sensitive to both myosin II and dynamin inhibition. The acto-myosin II rings therefore play a key role in constricting the neck of budding bulk endosomes before dynamin-dependent fission from the plasma membrane of neurosecretory cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.