Evidence map›Paper›PMID 25631803›Full record

ArticleJournal of clinical microbiology2015

Matrix-assisted laser desorption ionization-time of flight mass spectrometry for differentiation of the dimorphic fungal species Paracoccidioides brasiliensis and Paracoccidioides lutzii.

João Nobrega de Almeida, Gilda M B Del Negro, Rafaella C Grenfell, Monica S M Vidal, Danilo Y Thomaz, Dulce S Y de Figueiredo, Eduardo Bagagli, Luiz Juliano, Gil Benard

Abstract read
In one paragraph

Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Rapid identification of Sporothrix brasiliensis by MALDI-TOF MS directly from clinical cultures in an Amazonian epidemic setting.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026
    Article
  2. Review
  3. Review
  4. Article
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  8. Diagnosis of Pulmonary Infections Due to Endemic Fungi.Diagnostics (Basel, Switzerland) · 2021
    Review
  9. Article
  10. A New Duplex PCR-Assay for the Detection and Identification ofJournal of fungi (Basel, Switzerland) · 2021
    Article
  11. mBio · 2020
    Article
  12. Molecular Tools for Detection and Identification ofJournal of fungi (Basel, Switzerland) · 2020
    Review
  13. Article
  14. Review
  15. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

João Nobrega de AlmeidaCentral Laboratory Division-LIM03, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil Laboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil jnaj99@gmail.com.
Gilda M B Del NegroLaboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil.
Rafaella C GrenfellDepartment of Biophysics, Escola Paulista Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
Monica S M VidalLaboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil.
Danilo Y ThomazLaboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil.
Dulce S Y de FigueiredoLaboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil.
Eduardo BagagliDepartment of Microbiology and Immunology, Instituto de Biociências de Botucatu, Universidade Estadual Paulista Júlio de Mesquita Filho, Botucatu, Brazil.
Luiz JulianoDepartment of Biophysics, Escola Paulista Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
Gil BenardLaboratory of Medical Mycology-LIM53, Hospital das Clínicas FMUSP, Instituto de Medicina Tropical, Universidade de São Paulo, São Paulo, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Isolates of Paracoccidioides brasiliensis and Paracoccidioides lutzii, previously characterized by molecular techniques, were identified for the first time by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). All isolates were correctly identified, with log score values of >2.0. Thus, MALDI-TOF MS is a new tool for differentiating species of the genus Paracoccidioides.

Indexed as

DNA, FungalDNA, Ribosomal SpacerHSP70 Heat-Shock ProteinsParacoccidioidesPolymerase Chain ReactionRNA, FungalSpecies SpecificitySpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationDNA, FungalDNA, Ribosomal SpacerHSP70 Heat-Shock ProteinsRNA, Fungal

Identifiers

PMID25631803
PMCPMC4365217

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.