ArticleThe Journal of biological chemistry2015
Differential phosphorylation of a regulatory subunit of protein kinase CK2 by target of rapamycin complex 1 signaling and the Cdc-like kinase Kns1.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 34 citations in OpenAlex.
- The Central FacilitaTOR: Coordinating Transcription and Translation in Eukaryotes.International journal of molecular sciences · 2025Review
- Integration of protein sequence and protein-protein interaction data by hypergraph learning to identify novel protein complexes.Briefings in bioinformatics · 2024Article
- Hsp90-Mediated Multi-Drug Resistance in DNA Polymerase-Defective Strains ofJournal of fungi (Basel, Switzerland) · 2024Article
- Pleiotropic roles of LAMMER kinase, Lkh1 in stress responses and virulence ofFrontiers in cellular and infection microbiology · 2024Article
- Transcription by the Three RNA Polymerases under the Control of the TOR Signaling Pathway inBiomolecules · 2023Review
- Article
- A proteomic screen of Ty1 integrase partners identifies the protein kinase CK2 as a regulator of Ty1 retrotransposition.Mobile DNA · 2022Article
- Phosphorylation of FAM134C by CK2 controls starvation-induced ER-phagy.Science advances · 2022Article
- Article
- Review
- TORC1 signaling regulates cytoplasmic pH through Sir2 in yeast.Aging cell · 2020Article
- Multilayered regulation of TORC1-body formation in budding yeast.Molecular biology of the cell · 2019Article
- The Dual-Specificity LAMMER Kinase Affects Stress-Response and Morphological Plasticity in Fungi.Frontiers in cellular and infection microbiology · 2019Review
- Assembly of the small ribosomal subunit in yeast: mechanism and regulation.RNA (New York, N.Y.) · 2018Review
- Signaling to and from the RNA Polymerase III Transcription and Processing Machinery.Annual review of biochemistry · 2018Review
- Azole resistance in aThe Journal of biological chemistry · 2018Article
- Transcriptional control of yeast ribosome biogenesis: A multifaceted role for general regulatory factors.Transcription · 2017Article
- CKB1 is involved in abscisic acid and gibberellic acid signaling to regulate stress responses in Arabidopsis thaliana.Journal of plant research · 2017Article
- Tor1 and CK2 kinases control a switch between alternative ribosome biogenesis pathways in a growth-dependent manner.PLoS biology · 2017Article
- Transcriptional activation of lipogenesis by insulin requires phosphorylation of MED17 by CK2.Science signaling · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Transcriptional regulation of ribosome and tRNA synthesis plays a central role in determining protein synthetic capacity and is tightly controlled in response to nutrient availability and cellular stress. In Saccharomyces cerevisiae, the regulation of ribosome and tRNA synthesis was recently shown to involve the Cdc-like kinase Kns1 and the GSK-3 kinase Mck1. In this study, we explored additional roles for these conserved kinases in processes connected to the target of rapamycin complex 1 (TORC1). We conducted a synthetic chemical-genetic screen in a kns1Δ mck1Δ strain and identified many novel rapamycin-hypersensitive genes. Gene ontology analysis showed enrichment for TORC1-regulated processes (vesicle-mediated transport, autophagy, and regulation of cell size) and identified new connections to protein complexes including the protein kinase CK2. CK2 is considered to be a constitutively active kinase and in budding yeast, the holoenzyme comprises two regulatory subunits, Ckb1 and Ckb2, and two catalytic subunits, Cka1 and Cka2. We show that Ckb1 is differentially phosphorylated in vivo and that Kns1 mediates this phosphorylation when nutrients are limiting and under all tested stress conditions. We determined that the phosphorylation of Ckb1 does not detectably affect the stability of the CK2 holoenzyme but correlates with the reduced occupancy of Ckb1 on tRNA genes after rapamycin treatment. Thus, the differential occupancy of tRNA genes by CK2 is likely to modulate its activation of RNA polymerase III transcription. Our data suggest that TORC1, via its effector kinase Kns1, may regulate the association of CK2 with some of its substrates by phosphorylating Ckb1.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.