Evidence map›Paper›PMID 25630869›Full record

ArticleJournal of dental research2015

Upregulation of gingival tissue miR-200b in obese periodontitis subjects.

A Z Kalea, R Hoteit, J Suvan, R C Lovering, J Palmen, J A Cooper, V K Khodiyar, Z Harrington, S E Humphries, F D'Aiuto

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of dental research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 2 pooled it
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 2 syntheses or guidelines pooled it, 47 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Article
  5. Article
  6. International journal of molecular sciences · 2024
    Article
  7. Review
  8. MicroRNAs: The Missing Link between Hypertension and Periodontitis?International journal of molecular sciences · 2024
    Review
  9. Unique miRomics Expression Profiles inInternational journal of molecular sciences · 2023
    Article
  10. Article
  11. Article
  12. Review
  13. MicroRNAs: Harbingers and shapers of periodontal inflammation.Seminars in cell & developmental biology · 2022
    Review
  14. Article
  15. Review
  16. Review
  17. Article
  18. Article
  19. Article
  20. Ascorbic Acid: A New Player of Epigenetic Regulation in LPS-Oxidative medicine and cellular longevity · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

A Z KaleaCentre for Cardiovascular Genetics, Institute of Cardiovascular Science, University College London, London, UK a.kalea@ucl.ac.uk.
R HoteitCentre for Cardiovascular Genetics, Institute of Cardiovascular Science, University College London, London, UK.
J SuvanUnit of Periodontology, Eastman Dental Institute, University College London, London, UK.
R C LoveringCentre for Cardiovascular Genetics, Institute of Cardiovascular Science, University College London, London, UK.
J PalmenCentre for Cardiovascular Genetics, Institute of Cardiovascular Science, University College London, London, UK.
J A CooperCentre for Cardiovascular Genetics, Institute of Cardiovascular Science, University College London, London, UK.
V K KhodiyarCentre for Cardiovascular Genetics, Institute of Cardiovascular Science, University College London, London, UK.
Z HarringtonPeninsula School of Dentistry, Plymouth University, Plymouth, UK.
S E HumphriesCentre for Cardiovascular Genetics, Institute of Cardiovascular Science, University College London, London, UK.
F D'AiutoUnit of Periodontology, Eastman Dental Institute, University College London, London, UK.
University College London · GBEastman Dental Hospital · GBUniversity of Plymouth · GB

Funding

British Heart Foundation PG08/008British Heart Foundation RG/08/008/25291British Heart Foundation RG/13/5/30112British Heart Foundation SP/07/007/23671Department of HealthParkinson's UK G-1307
6 · The paper itself

Abstract

Increased local immune and inflammatory responses in obese individuals with periodontitis may explain the aggressive clinical presentation and altered treatment response when compared to that of normal weight subjects. Our goal was to identify any differences in microRNA (miRNA) expression profiles of gingival tissue in periodontitis when obesity is present, which may suggest novel molecular pathways that this miRNA network may affect. Total RNA was extracted from gingival tissue biopsies collected from normal weight and obese individuals with periodontitis; miRNA expression profiling was performed with Affymetrix GeneChip miRNA 3.0 arrays; and results were validated with quantitative reverse transcription polymerase chain reaction (qRT-PCR). In silico identification of previously confirmed miRNA gene targets was conducted through miRTarBase and miRWalk databases, and pathway enrichment analysis identified enriched miRNA gene sets. Expression of selected genes in the same biopsy samples was tested with qRT-PCR. The gingival tissue miRNA profile of obese patients, compared to that of normal weight patients, showed 13 upregulated and 22 downregulated miRNAs, among which miR-200b was validated by qRT-PCR to be significantly increased in obesity. Functional analysis of 51 experimentally validated miR-200b gene targets identified enrichment of genes involved in cell motility, differentiation, DNA binding, response to stimulus, and vasculature development pathways not previously identified in the obesity-specific disease profile. Furthermore, the expression of the miR-200b gene targets ZEB1/2, GATA2, and KDR was confirmed by qRT-PCR as being lower in obese patients with periodontitis versus normal weight patients, suggesting a role of miR-200b in regulation of a set of gene targets and biological pathways relevant to wound healing and angiogenesis. Functional studies to explore the role of miR-200b in the above processes may offer new insights on putative therapeutic targets for this group of patients.

Indexed as

AdultBody WeightCell DifferentiationCell MovementDNA-Binding ProteinsFemaleGATA2 Transcription FactorGene Expression ProfilingGingivaHomeodomain ProteinsHumansMaleMicroRNAsNeovascularization, PhysiologicObesityPeriodontitisDNA-Binding ProteinsGATA2 protein, humanGATA2 Transcription FactorHomeodomain ProteinsMicroRNAsMIRN200 microRNA, humanRepressor ProteinsTranscription FactorsVascular Endothelial Growth Factor Receptor-2ZEB1 protein, humanZEB2 protein, humanZinc Finger E-box-Binding Homeobox 1Zinc Finger E-box Binding Homeobox 2angiogenesisepithelialmicroRNA-200bobesityperiodontal diseasewound healing

Identifiers

PMID25630869
PMCPMC4541096
OpenAlexW2108984590

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.