ArticleIn vitro cellular & developmental biology. Animal2015
Differentiation of human skin-derived precursor cells into functional islet-like insulin-producing cell clusters.
Article in In vitro cellular & developmental biology. Animal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Extracellular vesicles of human transformed skin-derived precursors containing miR-221-3p promote hair growth through DKK2-mediated Wnt/Bioengineering & translational medicine · 2026Article
- Stem Cells Reprogramming in Diabetes Mellitus and Diabetic Complications: Recent Advances.Current diabetes reviews · 2025Review
- Facilitation of mouse skin-derived precursor growth and yield by optimizing plating density.Open life sciences · 2021Article
- Inflammation Alters the Secretome and Immunomodulatory Properties of Human Skin-Derived Precursor Cells.Cells · 2020Article
- The Human Skin-Derived Precursors for Regenerative Medicine: Current State, Challenges, and Perspectives.Stem cells international · 2018Review
- Rapid isolation and expansion of skin-derived precursor cells from human primary fibroblast cultures.Biology open · 2017Article
- FGF-2b and h-PL Transform Duct and Non-Endocrine Human Pancreatic Cells into Endocrine Insulin Secreting Cells by Modulating Differentiating Genes.International journal of molecular sciences · 2017Article
- Adult muscle-derived stem cells engraft and differentiate into insulin-expressing cells in pancreatic islets of diabetic mice.Stem cell research & therapy · 2017Article
- [Research progress of skin-derived precursor cells].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2017Review
- Stem cells - biological update and cell therapy progress.Clujul medical (1957) · 2015Review
- Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advances in cell-replacement strategies for diabetes have focused on renewable sources of glucose-responsive, insulin-producing cells (IPCs). One of the most proper alternatives is multipotent skin-derived precursors cells (SKPs), which can be differentiated into IPCs. In this study, we reported the isolation and expansion of human skin-derived precursors (hSKPs) followed by their differentiation into IPCs in vitro, through exposure to suitable differentiation factors. The gene expression of endocrine β cell markers was analyzed by reverse transcriptase-polymerase chain reaction. In addition, insulin production was examined immunocytochemically, and insulin and C-peptide secretion were examined using enzyme-linked immunosorbent assay. Dithizone-stained cellular clusters were observed after approximately 20 d. The clusters were found to be immunoreactive to insulin and expressed multiple genes related to pancreatic β cell development and function: insulin, Pdx-1, Islet-1, NeuroD, Glut-2, Pax-4, Ngn-3, and Nkx2.2, but not to other pancreas-specific hormones such as glucagon and somatostatin. Cellular clusters were also able to secrete detectable amounts of insulin and C-peptide in a glucose dose-dependent manner. These findings suggest that human SKPs can differentiate into functional IPCs. This may offer a safer cell source for future stem cell-based therapies.
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