ArticlePloS one2015
Decreasing NF-κB expression enhances odontoblastic differentiation and collagen expression in dental pulp stem cells exposed to inflammatory cytokines.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 51 citations in OpenAlex.
- Inflammation-Regeneration Axis of Dental Pulp Stem Cells: Wnt/NF-κB Crosstalk.International endodontic journal · 2026Review
- Mechanobiological and Molecular Alterations in the Aging Dentin-Pulp Complex.Life (Basel, Switzerland) · 2026Article
- MG132 facilitates dentin regeneration by modulating inflammation and odontoblast differentiation.Cell and tissue research · 2026Article
- The effect of fluocinolone acetonide, ProRoot MTA and their combination on inflammation and odontogenic differentiation of inflamed human dental pulp cells.Clinical oral investigations · 2025Article
- Effects triggered by tumor necrosis factor-α in immortalized murine dental pulp and pre-osteoblastic cells.Brazilian oral research · 2025Article
- The Biofunctional Monomer, the Calcium Salt of 4-Methacryloxyethyl Trimellitic Acid, Promotes Odontoblast Differentiation in Three-Dimensional Culture System.International journal of biomaterials · 2025Article
- Review
- Methylenetetrahydrofolate reductase polymorphisms in dental caries-induced pulp inflammation and regeneration of dentine-pulp complex: Future perspectives.The Saudi dental journal · 2023Article
- Evaluation of Current Studies to Elucidate Processes in Dental Follicle Cells Driving Osteogenic Differentiation.Biomedicines · 2023Review
- Effects of different signaling pathways on odontogenic differentiation of dental pulp stem cells: a review.Frontiers in physiology · 2023Review
- Therapeutic Potential of Synthetic HumanMediators of inflammation · 2022Article
- Activation of NF-κB signaling via cytosolic mitochondrial RNA sensing in kerotocytes with mitochondrial DNA common deletion.Scientific reports · 2021Article
- The NF-kB and Collagen Type 1 Expression in Dental Pulp after Treated Calcium Hydroxide Combined with Propolis.European journal of dentistry · 2021Article
- Microenvironment Influences Odontogenic Mesenchymal Stem Cells Mediated Dental Pulp Regeneration.Frontiers in physiology · 2021Review
- Shared Genetic and Epigenetic Mechanisms between the Osteogenic Differentiation of Dental Pulp Stem Cells and Bone Marrow Stem Cells.BioMed research international · 2021Article
- The Genetic and Epigenetic Mechanisms Involved in Irreversible Pulp Neural Inflammation.Disease markers · 2021Article
- The Effects of Biomaterial Implant Wear Debris on Osteoblasts.Frontiers in cell and developmental biology · 2020Review
- Odontoblast-like Cytodifferentiation of Dental Stem Cells: A Review.Iranian endodontic journal · 2020Review
- Persistent organic pollutants (POPs) increase rage signaling to promote downstream cardiovascular remodeling.Environmental toxicology · 2019Article
- Effect of canonical NF-κB signaling pathway on the differentiation of rat dental epithelial stem cells.Stem cell research & therapy · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Inflammatory response in the dental pulp can alter the collagen matrix formation by dental pulp stem cells and lead to a delay or poor healing of the pulp. This inflammatory response is mediated by cytokines, including interleukin-1β and tumor necrosis factor-α. In this study, it is hypothesized that suppressing the actions of these inflammatory cytokines by knocking down the activity of transcription factor Nuclear Factor-κB will lead to dental pulp stem cell differentiation into odontoblasts and the production of collagen. Here, the role of Nuclear Factor-κB signaling and its reduction was examined during odontogenic behavior in the presence of these cytokines. The results showed a significant increase in Nuclear Factor-κB gene expression and p65 protein expression by interleukin-1β and tumor necrosis factor-α. Nuclear Factor-κB activation in the presence of these cytokines decreased significantly in a dose-dependent manner by a Nuclear Factor-κB inhibitor (MG132) and p65 siRNA. Down-regulation of Nuclear Factor-κB activity also enhanced the gene expression of the odontoblastic markers (dentin sialophosphoprotein, Nestin, and alkaline phosphatase) and displayed an odontoblastic cell morphology indicating the promotion of odontogenic differentiation of dental pulp stem cells. Finally, dental pulp stem cells exposed to reduced Nuclear Factor-κB activity resulted in a significant increase in collagen (I)-α1 expression in the presence of these cytokines. In conclusion, a decrease in Nuclear Factor-κB in dental pulp stem cells in the presence of inflammatory cytokines enhanced odontoblastic differentiation and collagen matrix formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.