ArticleFrontiers in plant science2014
Identification of multiple salicylic acid-binding proteins using two high throughput screens.
Article in Frontiers in plant science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 143 citations in OpenAlex.
- Towards an integrated molecular understanding of plant hormones.Journal of integrative plant biology · 2026Review
- Molecular insights into volatile organic compound sensing and signaling in plants.The Plant journal : for cell and molecular biology · 2026Review
- Proteome and Ubiquitinome Analyses Reveal the Involvement of Ubiquitination in Resistance to Maize Lethal Necrosis.Molecular plant pathology · 2025Article
- Integrative multi-transcriptomic analysis uncovers core genes and potential defense mechanisms in rice-Magnoporthe oryzae interaction.Plant cell reports · 2025Article
- Characterization and expression patterns of theJournal of genetics · 2025Article
- Profiling sorghum-microbe interactions with a specialized photoaffinity probe identifies key sorgoleone binders inApplied and environmental microbiology · 2024Article
- Salicylic acid in plant immunity and beyond.The Plant cell · 2024Review
- The Underexplored Mechanisms of Wheat Resistance to Leaf Rust.Plants (Basel, Switzerland) · 2023Review
- Seeing is believing: Understanding functions of NPR1 and its paralogs in plant immunity through cellular and structural analyses.Current opinion in plant biology · 2023Review
- Shared and Related Molecular Targets and Actions of Salicylic Acid in Plants and Humans.Cells · 2023Review
- From prediction to function: Current practices and challenges towards the functional characterization of type III effectors.Frontiers in microbiology · 2023Review
- Analysis of the apoplast fluid proteome during the induction of systemic acquired resistance inPeerJ · 2023Article
- The role of photorespiration in plant immunity.Frontiers in plant science · 2023Review
- Arabidopsis Cys2/His2 Zinc Finger Transcription Factor ZAT18 Modulates the Plant Growth-Defense Tradeoff.International journal of molecular sciences · 2022Article
- Organelles and phytohormones: a network of interactions in plant stress responses.Journal of experimental botany · 2022Review
- The Mediator Complex: A Central Coordinator of Plant Adaptive Responses to Environmental Stresses.International journal of molecular sciences · 2022Review
- Article
- Non-Expresser of PR-Genes 1 Positively Regulates Abscisic Acid Signaling inPlants (Basel, Switzerland) · 2022Article
- cDNA Transcriptome ofInternational journal of molecular sciences · 2022Article
- An Anecdote on Prospective Protein Targets for Developing Novel Plant Growth Regulators.Molecular biotechnology · 2022Review
1 more citing papers are in PubMed but not listed here.
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Authors and funding
17 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salicylic acid (SA) is an important hormone involved in many diverse plant processes, including floral induction, stomatal closure, seed germination, adventitious root initiation, and thermogenesis. It also plays critical functions during responses to abiotic and biotic stresses. The role(s) of SA in signaling disease resistance is by far the best studied process, although it is still only partially understood. To obtain insights into how SA carries out its varied functions, particularly in activating disease resistance, two new high throughput screens were developed to identify novel SA-binding proteins (SABPs). The first utilized crosslinking of the photo-reactive SA analog 4-AzidoSA (4AzSA) to proteins in an Arabidopsis leaf extract, followed by immuno-selection with anti-SA antibodies and then mass spectroscopy-based identification. The second utilized photo-affinity crosslinking of 4AzSA to proteins on a protein microarray (PMA) followed by detection with anti-SA antibodies. To determine whether the candidate SABPs (cSABPs) obtained from these screens were true SABPs, recombinantly-produced proteins were generated and tested for SA-inhibitable crosslinking to 4AzSA, which was monitored by immuno-blot analysis, SA-inhibitable binding of the SA derivative 3-aminoethylSA (3AESA), which was detected by a surface plasmon resonance (SPR) assay, or SA-inhibitable binding of [(3)H]SA, which was detected by size exclusion chromatography. Based on our criteria that true SABPs must exhibit SA-binding activity in at least two of these assays, nine new SABPs are identified here; nine others were previously reported. Approximately 80 cSABPs await further assessment. In addition, the conflicting reports on whether NPR1 is an SABP were addressed by showing that it bound SA in all three of the above assays.
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