ArticleDental press journal of orthodontics
Protein biomarkers of external root resorption: a new protein extraction protocol. Are we going in the right direction?
Article in Dental press journal of orthodontics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Salivary and gingival crevicular fluid (GCF) protein biomarkers as proxies of root resorption following orthodontic tooth movement. A systematic review.Journal of clinical and experimental dentistry · 2025Review
- Tissue-specific biomarkers in gingival crevicular fluid are correlated with external root resorption caused by constant mechanical load: an in vivo study.Clinical oral investigations · 2021Article
- A Bioinformatics Systems Biology Analysis of the Current Oral Proteomic Biomarkers and Implications for Diagnosis and Treatment of External Root Resorption.International journal of molecular sciences · 2021Review
- Biomarker-based monitoring of orthodontically induced root resorption: Expression of cementum protein-1 in orthodontic patients with and without skeletal anchorage.Journal of oral and maxillofacial pathology : JOMFPArticle
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Authors and funding
1 author.
Funding
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Abstract
objectiveThe aim of this study is to determine a protocol of gingival crevicular fluid protein extraction used for the first dimension of 2-DE gels. It also aims at conducting a review on the current candidates for protein markers of this pathology, all of which may be used to prevent the disease.
methodsGingival crevicular fluid was collected from two groups of 60 patients each, with and without external root resorption. Samples were extracted by means of various methods of protein extraction. SDS-PAGE gels were used to assess the quality of the method which was subsequently tested during isoelectric focusing of 2-DE gels taken from samples of patients with and without the disease.
resultsMilli-Q ultrapure ice cold water, without precipitation for gingival crevicular fluid protein extraction, proved the method with greatest sharpness to detect protein bands. Additionally, it allowed two-dimensional electrophoresis to be performed.
conclusionThe new protein extraction protocol does not interfere in isoeletric focusing of 2-DE gels. Furthermore, it provides the greatest sharpness in detecting protein bands of SDS-PAGE gels. This will allow mapping and searching of new external root resorption markers, particularly due to the difficulty in carrying out molecular tests with the current candidates for protein markers.
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