ArticleJournal of tissue engineering and regenerative medicine2017
Human chondrocyte migration behaviour to guide the development of engineered cartilage.
Article in Journal of tissue engineering and regenerative medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Dynamic Col-HZ Hydrogel with efficient delivery of bioactivator promotes ECM deposition and cartilage formation.Materials today. Bio · 2025Article
- Electrical stimulation for cartilage tissue engineering - A critical review from an engineer's perspective.Heliyon · 2024Review
- Effect of Passaging on Bovine Chondrocyte Gene Expression and Engineered Cartilage Production.Tissue engineering. Part A · 2024Article
- Addition of collagen type I in agarose created a dose-dependent effect on matrix production in engineered cartilage.Regenerative biomaterials · 2022Article
- Probing Multicellular Tissue Fusion of Cocultured Spheroids-A 3D-Bioassembly Model.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Recellularization and Integration of Dense Extracellular Matrix by Percolation of Tissue Microparticles.Advanced functional materials · 2021Article
- Endogenous Repair and Regeneration of Injured Articular Cartilage: A Challenging but Promising Therapeutic Strategy.Aging and disease · 2021Review
- Electric field stimulation for tissue engineering applications.BMC biomedical engineering · 2021Review
- Pulsed electromagnetic fields promote repair of focal articular cartilage defects with engineered osteochondral constructs.Biotechnology and bioengineering · 2020Article
- Transcriptome analyses identify key genes and potential mechanisms in a rat model of osteoarthritis.Journal of orthopaedic surgery and research · 2018Article
- Transient expression of the diseased phenotype of osteoarthritic chondrocytes in engineered cartilage.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2017Article
- Article
- Nutrient Channels Aid the Growth of Articular Surface-Sized Engineered Cartilage Constructs.Tissue engineering. Part A · 2016Article
- Optimizing nutrient channel spacing and revisiting TGF-beta in large engineered cartilage constructs.Journal of biomechanics · 2016Article
- In vitro effects of platelet-rich gel supernatants on histology and chondrocyte apoptosis scores, hyaluronan release and gene expression of equine cartilage explants challenged with lipopolysaccharide.BMC veterinary research · 2016Article
- High seeding density of human chondrocytes in agarose produces tissue-engineered cartilage approaching native mechanical and biochemical properties.Journal of biomechanics · 2016Article
- Dexamethasone Release from Within Engineered Cartilage as a Chondroprotective Strategy Against Interleukin-1α.Tissue engineering. Part A · 2016Article
- Tissue Engineering a Biological Repair Strategy for Lumbar Disc Herniation.BioResearch open access · 2015Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
Tissue-engineering techniques have been successful in developing cartilage-like tissues in vitro using cells from animal sources. The successful translation of these strategies to the clinic will likely require cell expansion to achieve sufficient cell numbers. Using a two-dimensional (2D) cell migration assay to first identify the passage at which chondrocytes exhibited their greatest chondrogenic potential, the objective of this study was to determine a more optimal culture medium for developing three-dimensional (3D) cartilage-like tissues using human cells. We evaluated combinations of commonly used growth factors that have been shown to promote chondrogenic growth and development. Human articular chondrocytes (AC) from osteoarthritic (OA) joints were cultured in 3D environments, either in pellets or encapsulated in agarose. The effect of growth factor supplementation was dependent on the environment, such that matrix deposition differed between the two culture systems. ACs in pellet culture were more responsive to bone morphogenetic protein (BMP2) alone or combinations containing BMP2 (i.e. BMP2 with PDGF or FGF). However, engineered cartilage development within agarose was better for constructs cultured with TGFβ3. These results with agarose and pellet culture studies set the stage for the development of conditions appropriate for culturing 3D functional engineered cartilage for eventual use in human therapies. Copyright © 2015 John Wiley & Sons, Ltd.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.