ArticleScientific reports2015
Simulated microgravity inhibits L-type calcium channel currents partially by the up-regulation of miR-103 in MC3T3-E1 osteoblasts.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 48 citations in OpenAlex.
- Bed rest and endurance retraining alter persistent inward current estimates in young and older males.Frontiers in physiology · 2026Article
- The hierarchical stratum response system of organism to microgravity during spaceflight.Military Medical Research · 2026Review
- Non-coding RNAs mediate mechanical load-regulating bone metabolic homeostasis.Frontiers in cell and developmental biology · 2025Review
- Mechanical loading regulates osteogenic differentiation and bone formation by modulating non-coding RNAs.PeerJ · 2025Review
- Article
- Cellular and Molecular Effects of Microgravity on the Immune System: A Focus on Bioactive Lipids.Biomolecules · 2024Review
- Phylobone: a comprehensive database of bone extracellular matrix proteins in human and model organisms.Bone research · 2023Article
- Examining Mechanisms for Voltage-Sensitive Calcium Channel-Mediated Secretion Events in Bone Cells.Calcified tissue international · 2023Review
- Protection of primary cilia is an effective countermeasure against the impairment of osteoblast function induced by simulated microgravity.Journal of cellular and molecular medicine · 2023Article
- Changes in interstitial fluid flow, mass transport and the bone cell response in microgravity and normogravity.Bone research · 2022Review
- Fluid Shear Stress Promotes Osteoblast Proliferation and Suppresses Mitochondrial-Mediated Osteoblast Apoptosis Through the miR-214-3p-ATF4 Signaling Axis.Physiological research · 2022Article
- Update on the effects of microgravity on the musculoskeletal system.NPJ microgravity · 2021Review
- MiR-103-3p targets the mAging cell · 2021Article
- Interactions between Muscle and Bone-Where Physics Meets Biology.Biomolecules · 2020Review
- Effect of miR-27b-5p on apoptosis of human vascular endothelial cells induced by simulated microgravity.Apoptosis : an international journal on programmed cell death · 2020Article
- Spaceflight-Induced Bone Tissue Changes that Affect Bone Quality and Increase Fracture Risk.Current osteoporosis reports · 2020Review
- MiR-291a-3p regulates the BMSCs differentiation via targeting DKK1 in dexamethasone-induced osteoporosis.The Kaohsiung journal of medical sciences · 2020Article
- miR-181c-5p mediates simulated microgravity-induced impaired osteoblast proliferation by promoting cell cycle arrested in the GJournal of cellular and molecular medicine · 2019Article
- Regulator of G Protein Signaling Protein 12 (Rgs12) Controls Mouse Osteoblast Differentiation via Calcium Channel/Oscillation and Gαi-ERK Signaling.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2019Article
- Gene Expression in Osteoblasts and Osteoclasts Under Microgravity Conditions: A Systematic Review.Current genomics · 2019Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
L-type voltage-sensitive calcium channels (LTCCs), particularly Cav1.2 LTCCs, play fundamental roles in cellular responses to mechanical stimuli in osteoblasts. Numerous studies have shown that mechanical loading promotes bone formation, whereas the removal of this stimulus under microgravity conditions results in a reduction in bone mass. However, whether microgravity exerts an influence on LTCCs in osteoblasts and whether this influence is a possible mechanism underlying the observed bone loss remain unclear. In the present study, we demonstrated that simulated microgravity substantially inhibited LTCC currents and suppressed Cav1.2 at the protein level in MC3T3-E1 osteoblast-like cells. In addition, reduced Cav1.2 protein levels decreased LTCC currents in MC3T3-E1 cells. Moreover, simulated microgravity increased miR-103 expression. Cav1.2 expression and LTCC current densities both significantly increased in cells that were transfected with a miR-103 inhibitor under mechanical unloading conditions. These results suggest that simulated microgravity substantially inhibits LTCC currents in osteoblasts by suppressing Cav1.2 expression. Furthermore, the down-regulation of Cav1.2 expression and the inhibition of LTCCs caused by mechanical unloading in osteoblasts are partially due to miR-103 up-regulation. Our study provides a novel mechanism for microgravity-induced detrimental effects on osteoblasts, offering a new avenue to further investigate the bone loss induced by microgravity.
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