ArticleScientific reports2015
Reference gene selection for cross-species and cross-ploidy level comparisons in Chrysanthemum spp.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Identification of theFrontiers in plant science · 2026Article
- Cross-species stability of reference genes in medicinal plants Arnica montana and Arnica chamissonis.Scientific reports · 2024Article
- Transcriptome and metabolome profiling unveil the accumulation of chlorogenic acid in autooctoploid Gongju.Frontiers in plant science · 2024Article
- CmNAC25 targets CmMYB6 to positively regulate anthocyanin biosynthesis during the post-flowering stage in chrysanthemum.BMC biology · 2023Article
- Selection of suitable reference genes for qPCR normalization in different developmental stages ofFrontiers in plant science · 2023Article
- Validation of Reference Genes in a Population of BlueberryPlants (Basel, Switzerland) · 2022Article
- Evaluation of Reference Genes in the Polyploid ComplexPlants (Basel, Switzerland) · 2022Article
- An Eruption of LTR Retrotransposons in the Autopolyploid Genomes ofPlants (Basel, Switzerland) · 2022Article
- Transcriptome analysis of differentially expressed genes in chrysanthemumPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Selection and validation of appropriate reference genes for RT-qPCR analysis of flowering stages and different genotypes of Iris germanica L.Scientific reports · 2021Article
- Validation of reference genes for quantitative gene expression in the Lippia alba polyploid complex (Verbenaceae).Molecular biology reports · 2021Article
- Selection and validation of reference genes for quantitative gene expression normalization in Taxus spp.Scientific reports · 2020Article
- Estimation of Molecular Pairwise Relatedness in Autopolyploid Crops.G3 (Bethesda, Md.) · 2020Article
- Selection of optimal reference genes for qRT-PCR analysis of shoot development and graviresponse in prostrate and erect chrysanthemums.PloS one · 2019Article
- Selection of Reference Genes for Expression Analysis in Chinese Medicinal HerbFrontiers in pharmacology · 2019Article
- Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya.Plant methods · 2019Article
- The Constitutive Expression of a Chrysanthemum ERF Transcription Factor Influences Flowering Time in Arabidopsis thaliana.Molecular biotechnology · 2019Article
- Selection and Validation of Reference Genes for mRNA Expression by Quantitative Real-Time PCR Analysis in Neolamarckia cadamba.Scientific reports · 2018Article
- Whole genome duplication enhances the photosynthetic capacity of Chrysanthemum nankingense.Molecular genetics and genomics : MGG · 2017Article
- Gibberellic Acid Signaling Is Required to Induce Flowering of Chrysanthemums Grown under Both Short and Long Days.International journal of molecular sciences · 2017Article
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5 authors.
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Abstract
The establishment of a (set of) stably expressed reference gene(s) is required to normalize transcription data. Polyploidy is very common in the plant kingdom, but it is not necessarily the case that a reference gene which works well at the diploid level will also work well at the polyploid level. Here, ten candidate reference genes are compared in the context of gene transcription in the genus Chrysanthemum. The robustness of some, but not all, of these was shown to be high across ploidy levels. MTP (metalloprotease) and ACTIN (actin) were the most stable in diploid and tetraploid C. nankingense, while PSAA (photosynthesis-related plastid gene representing photosystem I) and EF-1α (elongation factor-1α) were the most stable in tetraploid and hexaploid C. zawadskii. EF-1α and PGK (phosphoglycerate kinase) was the best combination for the complete set of four taxa. These results suggest that when making cross-species comparison of transcript abundance involving different ploidy levels, care needs to be taken in the selection of reference gene(s).
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