ArticleThe Journal of biological chemistry2015
G-quadruplexes significantly stimulate Pif1 helicase-catalyzed duplex DNA unwinding.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 47 citations in OpenAlex.
- Dynamic Assemblies in Genome Maintenance.Advances in experimental medicine and biology · 2026Review
- The Role of SF1 and SF2 Helicases in Biotechnological Applications.Applied biochemistry and biotechnology · 2024Review
- Pif1 Activity is Modulated by DNA Sequence and Structure.Biochemistry · 2022Article
- Article
- Impact of G-Quadruplexes on the Regulation of Genome Integrity, DNA Damage and Repair.Biomolecules · 2021Review
- G-quadruplex targeting chemical nucleases as a nonperturbative tool for analysis of cellular G-quadruplex DNA.iScience · 2021Article
- Mgs1 function at G-quadruplex structures during DNA replication.Current genetics · 2021Review
- The Cellular Functions and Molecular Mechanisms of G-Quadruplex Unwinding Helicases in Humans.Frontiers in molecular biosciences · 2021Review
- Review
- Pif1 family DNA helicases: A helpmate to RNase H?DNA repair · 2019Review
- Structural and functional analysis of the nucleotide and DNA binding activities of the human PIF1 helicase.Nucleic acids research · 2019Article
- Non-duplex G-Quadruplex Structures Emerge as Mediators of Epigenetic Modifications.Trends in genetics : TIG · 2019Review
- Pif1 helicase unfolding of G-quadruplex DNA is highly dependent on sequence and reaction conditions.The Journal of biological chemistry · 2018Article
- Insights into the structural and mechanistic basis of multifunctional S. cerevisiae Pif1p helicase.Nucleic acids research · 2018Article
- Structure and function of Pif1 helicase.Biochemical Society transactions · 2017Review
- The functions of the multi-tasking Pfh1Current genetics · 2017Review
- A helical bundle in the N-terminal domain of the BLM helicase mediates dimer and potentially hexamer formation.The Journal of biological chemistry · 2017Article
- Review
- Structure, Properties, and Biological Relevance of the DNA and RNA G-Quadruplexes: Overview 50 Years after Their Discovery.Biochemistry. Biokhimiia · 2016Article
- G4IPDB: A database for G-quadruplex structure forming nucleic acid interacting proteins.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The evolutionarily conserved G-quadruplexes (G4s) are faithfully inherited and serve a variety of cellular functions such as telomere maintenance, gene regulation, DNA replication initiation, and epigenetic regulation. Different from the Watson-Crick base-pairing found in duplex DNA, G4s are formed via Hoogsteen base pairing and are very stable and compact DNA structures. Failure of untangling them in the cell impedes DNA-based transactions and leads to genome instability. Cells have evolved highly specific helicases to resolve G4 structures. We used a recombinant nuclear form of Saccharomyces cerevisiae Pif1 to characterize Pif1-mediated DNA unwinding with a substrate mimicking an ongoing lagging strand synthesis stalled by G4s, which resembles a replication origin and a G4-structured flap in Okazaki fragment maturation. We find that the presence of G4 may greatly stimulate the Pif1 helicase to unwind duplex DNA. Further studies reveal that this stimulation results from G4-enhanced Pif1 dimerization, which is required for duplex DNA unwinding. This finding provides new insights into the properties and functions of G4s. We discuss the observed activation phenomenon in relation to the possible regulatory role of G4s in the rapid rescue of the stalled lagging strand synthesis by helping the replicator recognize and activate the replication origin as well as by quickly removing the G4-structured flap during Okazaki fragment maturation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.