ArticleJournal of microscopy2015
Developing 3D SEM in a broad biological context.
Article in Journal of microscopy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
51 citing papers in PubMed, 105 citations in OpenAlex.
- Mitochondria‑endoplasmic reticulum contact site nexus: Molecular integration, disease pathogenesis and therapeutic opportunities (Review).International journal of molecular medicine · 2026Review
- Advanced imaging of membrane contact sites.Nature cell biology · 2026Review
- RNA-based CLEM (RCLEM) bridging RNA localisation and ultrastructural mapping in 3D.Journal of microscopy · 2026Article
- Electron microscopy-based three-dimensional subcellular imaging of plant male gametophyte.Journal of integrative plant biology · 2026Review
- Advances in tissue optical clearing for 3D imaging in large animal.Frontiers of optoelectronics · 2025Review
- New Approaches Based on Serial-Block Face Electron Microscopy to Investigate the Peripheral Nervous System.Journal of the peripheral nervous system : JPNS · 2025Article
- Repetitive ultramicrotome trimming and SEM imaging for characterizing printed multilayer structures.Scientific reports · 2024Article
- Split-Luciferase Reassembly Assay to Measure Endoplasmic Reticulum-Mitochondria Contacts in Live Cells.Journal of visualized experiments : JoVE · 2024Article
- The desmosome comes into focus.The Journal of cell biology · 2024Review
- Sample preparation and data collection for serial block face scanning electron microscopy of mammalian cell monolayers.PloS one · 2024Article
- Advanced Imaging Techniques for the Characterization of Subcellular Organelle Structure in Pancreatic Islet β Cells.Comprehensive Physiology · 2023Article
- Morphological changes in the anterior vaginal wall caused by aging: a scanning electron microscopy study.International urogynecology journal · 2023Article
- How innovations in methodology offer new prospects for volume electron microscopy.Journal of microscopy · 2022Review
- Soft X-ray tomography to map and quantify organelle interactions at the mesoscale.Structure (London, England : 1993) · 2022Article
- One for All, All for One: A Close Look at In-Resin Fluorescence Protocols for CLEM.Frontiers in cell and developmental biology · 2022Review
- Focused Ion Beam-Scanning Electron Microscopy for Investigating Plasmodesmal Densities.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Reactive oxygen FIB spin milling enables correlative workflow for 3D super-resolution light microscopy and serial FIB/SEM of cultured cells.Scientific reports · 2021Article
- 3D reconstruction of structures of hatched larva and young juvenile of the larvacean Oikopleura dioica using SBF-SEM.Scientific reports · 2021Article
- Protocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insects.Scientific reports · 2021Article
- Volume electron microscopy: analyzing the lung.Histochemistry and cell biology · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
22 authors at 8 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When electron microscopy (EM) was introduced in the 1930s it gave scientists their first look into the nanoworld of cells. Over the last 80 years EM has vastly increased our understanding of the complex cellular structures that underlie the diverse functions that cells need to maintain life. One drawback that has been difficult to overcome was the inherent lack of volume information, mainly due to the limit on the thickness of sections that could be viewed in a transmission electron microscope (TEM). For many years scientists struggled to achieve three-dimensional (3D) EM using serial section reconstructions, TEM tomography, and scanning EM (SEM) techniques such as freeze-fracture. Although each technique yielded some special information, they required a significant amount of time and specialist expertise to obtain even a very small 3D EM dataset. Almost 20 years ago scientists began to exploit SEMs to image blocks of embedded tissues and perform serial sectioning of these tissues inside the SEM chamber. Using first focused ion beams (FIB) and subsequently robotic ultramicrotomes (serial block-face, SBF-SEM) microscopists were able to collect large volumes of 3D EM information at resolutions that could address many important biological questions, and do so in an efficient manner. We present here some examples of 3D EM taken from the many diverse specimens that have been imaged in our core facility. We propose that the next major step forward will be to efficiently correlate functional information obtained using light microscopy (LM) with 3D EM datasets to more completely investigate the important links between cell structures and their functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.