ArticleAlcoholism, clinical and experimental research2015
Alcohol intoxication following muscle contraction in mice decreases muscle protein synthesis but not mTOR signal transduction.
Article in Alcoholism, clinical and experimental research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- The combined association of physical activity and alcohol use with long-term mortality: an age-stratified analysis.BMC public health · 2024Article
- Effects of chronic alcohol intoxication on aerobic exercise-induced adaptations in female mice.Journal of applied physiology (Bethesda, Md. : 1985) · 2024Article
- Alcohol, Resistance Exercise, and mTOR Pathway Signaling: An Evidence-Based Narrative Review.Biomolecules · 2022Review
- The sex-specific prognostic utility of sarcopenia in cirrhosis.Journal of cachexia, sarcopenia and muscle · 2022Review
- Cellular Bioenergetics: Experimental Evidence for Alcohol-induced Adaptations.Function (Oxford, England) · 2022Review
- The Prophylactic Effects of Glutamine on Muscle Protein Synthesis and Degradation in Rats with Ethanol-Induced Liver Damage.Nutrients · 2021Article
- Ethanol acutely antagonizes the refeeding-induced increase in mTOR-dependent protein synthesis and decrease in autophagy in skeletal muscle.Molecular and cellular biochemistry · 2019Article
- Inhibition of Hepatotoxicity by a LXR Inverse Agonist in a Model of Alcoholic Liver Disease.ACS pharmacology & translational science · 2018Article
- Physiological processes underlying organ injury in alcohol abuse.American journal of physiology. Endocrinology and metabolism · 2016Review
- Loss of REDD1 augments the rate of the overload-induced increase in muscle mass.American journal of physiology. Regulatory, integrative and comparative physiology · 2016Article
- Emerging role for regulated in development and DNA damage 1 (REDD1) in the regulation of skeletal muscle metabolism.American journal of physiology. Endocrinology and metabolism · 2016Review
- Acute Alcohol-Induced Decrease in Muscle Protein Synthesis in Female Mice Is REDD-1 and mTOR-Independent.Alcohol and alcoholism (Oxford, Oxfordshire) · 2016Article
- Article
- Dysregulation of skeletal muscle protein metabolism by alcohol.American journal of physiology. Endocrinology and metabolism · 2015Review
- Convergence of theories of alcohol administration postanabolic stimulation on mTOR signaling: lessons for exercise regimen.Alcoholism, clinical and experimental research · 2015Article
- Moderate alcohol consumption does not impair overload-induced muscle hypertrophy and protein synthesis.Physiological reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAlcohol (ethanol [EtOH]) intoxication antagonizes stimulation of muscle protein synthesis and mammalian target of rapamycin (mTOR) signaling. However, whether the anabolic response can be reversed when alcohol is consumed after the stimulus is unknown.
methodsA single bout of electrically stimulated muscle contractions (10 sets of 6 contractions) was induced in fasted male C57BL/6 mice 2 hours prior to alcohol intoxication. EtOH was injected intraperitoneally (3 g/kg), and the gastrocnemius/plantaris muscle complex was collected 2 hours later from the stimulated and contralateral unstimulated control leg.
resultsMuscle contraction increased protein synthesis 28% in control mice, while EtOH abolished this stimulation-induced increase. Further, EtOH suppressed the rate of synthesis ~75% compared to control muscle irrespective of stimulation. This decrease was associated with impaired protein elongation as EtOH increased the phosphorylation of eEF2 Thr(56) . In contrast, stimulation-induced increases in mTOR protein complex-1 (mTORC1) (S6K1 Thr(421) /Ser(424) , S6K1 Thr(389) , rpS6 Ser(240/244) , and 4E-BP1 Thr(37/46) ) and mitogen-activated protein kinase (MAPK) (JNK Thr(183) /Tyr(185) , p38 Thr(180) /Tyr(182) , and rpS6S(235/236) ) signaling were not reversed by acute EtOH.
conclusionsThese data suggest that EtOH-induced decreases in protein synthesis in fasted mice may be independent of mTORC1 and MAPK signaling following muscle contraction and instead due to the antagonistic actions of EtOH on mRNA translation elongation. Therefore, EtOH suppresses the contraction-induced increase in protein synthesis, and over time has the potential to prevent skeletal muscle hypertrophy induced by repeated muscle contraction.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.