ArticleScientific reports2015
Functional characterization of a vanillin dehydrogenase in Corynebacterium glutamicum.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 53 citations in OpenAlex.
- Two routes for tyrosol production by metabolic engineering of Corynebacterium glutamicum.Biotechnology for biofuels and bioproducts · 2025Article
- Studies on Square Wave and Cyclic Voltammetric Behavior of 1,2- and 1,4-Dihydroxybenzenes and Their Derivatives in Acetic Acid, Ethyl Acetate and Mixtures of the Two.Methods and protocols · 2024Article
- Utilization of orange peel waste for sustainable amino acid production byFrontiers in bioengineering and biotechnology · 2024Article
- Metabolic engineering of Corynebacterium glutamicum for fatty alcohol production from glucose and wheat straw hydrolysate.Biotechnology for biofuels and bioproducts · 2023Article
- Small protein Cgl2215 enhances phenolic tolerance by promoting MytA activity in Corynebacterium glutamicum.Stress biology · 2022Article
- The Catabolic System of Acetovanillone and Acetosyringone inApplied and environmental microbiology · 2022Article
- Application of Corynebacterium glutamicum engineering display system in three generations of biorefinery.Microbial cell factories · 2022Review
- EngineeredFrontiers in bioengineering and biotechnology · 2022Article
- Article
- Vanillic acid and methoxyhydroquinone production from guaiacyl units and related aromatic compounds using Aspergillus niger cell factories.Microbial cell factories · 2021Article
- A Timed Off-Switch for Dynamic Control of Gene Expression inFrontiers in bioengineering and biotechnology · 2021Article
- Tolerance and transcriptional analysis of Corynebacterium glutamicum on biotransformation of toxic furaldehyde and benzaldehyde inhibitory compounds.Journal of industrial microbiology & biotechnology · 2019Article
- Inhibitory Effect of Volatiles Emitted FromFrontiers in microbiology · 2019Article
- Molecular cloning and characterization of vanillin dehydrogenase from Streptomyces sp. NL15-2K.BMC microbiology · 2018Article
- Biotransformation of ferulic acid to protocatechuic acid by Corynebacterium glutamicum ATCC 21420 engineered to express vanillate O-demethylase.AMB Express · 2017Article
- A bacterial aromatic aldehyde dehydrogenase critical for the efficient catabolism of syringaldehyde.Scientific reports · 2017Article
- Evolutionary, computational, and biochemical studies of the salicylaldehyde dehydrogenases in the naphthalene degradation pathway.Scientific reports · 2017Article
- Discovery of piperonal-converting oxidase involved in the metabolism of a botanical aromatic aldehyde.Scientific reports · 2016Article
- Characterization of a Unique Pathway for 4-Cresol Catabolism Initiated by Phosphorylation in Corynebacterium glutamicum.The Journal of biological chemistry · 2016Article
- Global Transcriptomic Analysis of the Response of Corynebacterium glutamicum to Vanillin.PloS one · 2016Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vanillin dehydrogenase (VDH) is a crucial enzyme involved in the degradation of lignin-derived aromatic compounds. Herein, the VDH from Corynebacterium glutamicum was characterized. The relative molecular mass (Mr) determined by SDS-PAGE was ~51 kDa, whereas the apparent native Mr values revealed by gel filtration chromatography were 49.5, 92.3, 159.0 and 199.2 kDa, indicating the presence of dimeric, trimeric and tetrameric forms. Moreover, the enzyme showed its highest level of activity toward vanillin at pH 7.0 and 30°C, and interestingly, it could utilize NAD(+) and NADP(+) as coenzymes with similar efficiency and showed no obvious difference toward NAD(+) and NADP(+). In addition to vanillin, this enzyme exhibited catalytic activity toward a broad range of substrates, including p-hydroxybenzaldehyde, 3,4-dihydroxybenzaldehyde, o-phthaldialdehyde, cinnamaldehyde, syringaldehyde and benzaldehyde. Conserved catalytic residues or putative cofactor interactive sites were identified based on sequence alignment and comparison with previous studies, and the function of selected residues were verified by site-directed mutagenesis analysis. Finally, the vdh deletion mutant partially lost its ability to grow on vanillin, indicating the presence of alternative VDH(s) in Corynebacterium glutamicum. Taken together, this study contributes to understanding the VDH diversity from bacteria and the aromatic metabolism pathways in C. glutamicum.
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