ArticleLab on a chip2015
Hydrogel-droplet microfluidic platform for high-resolution imaging and sorting of early larval Caenorhabditis elegans.
Article in Lab on a chip, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed.
- Integrated microfluidic biosensors: shaping the future of quantitative life sciences and on-chip molecular diagnostics.Lab on a chip · 2026Review
- Integrated Microfluidic Platform for Longitudinal Imaging, Functional Assays, and Downstream Proteomics in C. elegans.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Automated Platforms inMicromachines · 2025Review
- Demand-driven active droplet generation and sorting based on positive pressure-controlled fluid wall.Analytical and bioanalytical chemistry · 2023Article
- A spiral microfluidic device for rapid sorting, trapping, and long-term live imaging ofMicrosystems & nanoengineering · 2023Article
- Microfluidic-AssistedCyborg and bionic systems (Washington, D.C.) · 2023Article
- Microfluidics for understanding model organisms.Nature communications · 2022Review
- Automated and Dynamic Control of Chemical Content in Droplets for Scalable Screens of Small Animals.Small (Weinheim an der Bergstrasse, Germany) · 2022Article
- Microswimmer Combing: Controlling Interfacial Dynamics for Open-Surface Multifunctional Screening of Small Animals.Advanced healthcare materials · 2021Article
- Microfluidic approaches forAnimal cells and systems · 2020Review
- Fluorescence-based sorting of Caenorhabditis elegans via acoustofluidics.Lab on a chip · 2020Article
- Imaging-assisted hydrogel formation for single cell isolation.Scientific reports · 2020Article
- Biomedical Application of Functional Materials in Organ-on-a-Chip.Frontiers in bioengineering and biotechnology · 2020Review
- Microfluidic Technologies for High Throughput Screening Through Sorting and On-Chip Culture ofMolecules (Basel, Switzerland) · 2019Review
- Pluronic gel-based burrowing assay for rapid assessment of neuromuscular health in C. elegans.Scientific reports · 2019Article
- Oscillating dispersed-phase co-flow microfluidic droplet generation: Multi-droplet size effect.Biomicrofluidics · 2018Article
- Microfluidics-enabled phenotyping of a whole population ofMicrosystems & nanoengineering · 2018Article
- Article
- Rapid and gentle hydrogel encapsulation of living organisms enables long-term microscopy over multiple hours.Communications biology · 2018Article
- Flexible and disposable paper- and plastic-based gel micropads for nematode handling, imaging, and chemical testing.APL bioengineering · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The nematode Caenorhabditis elegans is an important model organism in research on neuroscience and development because of its stereotyped anatomy, relevance to human biology, and ease of culture and genetic manipulation. The first larval stage (L1) is of particular interest in many biological problems, including post-embryonic developmental processes and developmental decision-making, such as dauer formation. However, L1's small size and high mobility make it difficult to manipulate; particularly in microfluidic chips, which have been used to great advantage in handling larger larvae and adult animals, small features are difficult to fabricate and these structures often get clogged easily, making the devices less robust. We have developed a microfluidic device to overcome these challenges and enable high-resolution imaging and sorting of early larval stage C. elegans via encapsulation in droplets of a thermosensitive hydrogel. To achieve precise handling of early larval stage worms, we demonstrated on-chip production, storage, and sorting of hydrogel droplets. We also demonstrated temporary immobilization of the worms within the droplets, allowing high-resolution imaging with minimal physiological perturbations. Because of the ability to array hydrogel droplets for handling a large number of L1 worms in a robust way, we envision that this platform will be widely applicable to screening in various developmental studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.