ArticleBioTechniques. Rapid dispatches2012
Short-read, high-throughput sequencing technology for STR genotyping.
Article in BioTechniques. Rapid dispatches, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 94 citations in OpenAlex.
- STRsensor: a computationally efficient method for STR allele-typing from massively parallel sequencing data.Briefings in bioinformatics · 2024Article
- Short tandem repeat profiling via next-generation sequencing for cell line authentication.Disease models & mechanisms · 2023Article
- A cost-benefit analysis for use of large SNP panels and high throughput typing for forensic investigative genetic genealogy.International journal of legal medicine · 2023Article
- Evaluation of Library Preparation Workflows and Applications to Different Sample Types Using the PowerSeqGenes · 2023Article
- Next-Generation Sequencing: ForenSeq™ DNA Signature Prep Kit with the Illumina MiSeq FGx.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Applications of massively parallel sequencing in forensic genetics.Genetics and molecular biology · 2022Article
- Development of SSR Databases Available for Both NGS and Capillary Electrophoresis in Apple, Pear and Tea.Plants (Basel, Switzerland) · 2021Article
- Enhanced mixture interpretation with macrohaplotypes based on long-read DNA sequencing.International journal of legal medicine · 2021Article
- Article
- BigFiRSt: A Software Program Using Big Data Technique for Mining Simple Sequence Repeats From Large-Scale Sequencing Data.Frontiers in big data · 2021Article
- Article
- Advancing precision medicine with personalized drug screening.Drug discovery today · 2019Review
- WHY WE FEAR GENETIC INFORMANTS: USING GENETIC GENEALOGY TO CATCH SERIAL KILLERS.The Columbia science and technology law review · 2019Article
- Massively parallel sequencing techniques for forensics: A review.Electrophoresis · 2018Review
- CRISPR-Cas9-targeted fragmentation and selective sequencing enable massively parallel microsatellite analysis.Nature communications · 2017Article
- A preliminary assessment of the ForenSeq™ FGx System: next generation sequencing of an STR and SNP multiplex.International journal of legal medicine · 2017Article
- A technique for setting analytical thresholds in massively parallel sequencing-based forensic DNA analysis.PloS one · 2017Article
- Evaluation of forensic DNA mixture evidence: protocol for evaluation, interpretation, and statistical calculations using the combined probability of inclusion.BMC genetics · 2016Article
- Sequence variation of 22 autosomal STR loci detected by next generation sequencing.Forensic science international. Genetics · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
18 authors at 2 institutions in 1 country.
Funding
Abstract
DNA-based methods for human identification principally rely upon genotyping of short tandem repeat (STR) loci. Electrophoretic-based techniques for variable-length classification of STRs are universally utilized, but are limited in that they have relatively low throughput and do not yield nucleotide sequence information. High-throughput sequencing technology may provide a more powerful instrument for human identification, but is not currently validated for forensic casework. Here, we present a systematic method to perform high-throughput genotyping analysis of the Combined DNA Index System (CODIS) STR loci using short-read (150 bp) massively parallel sequencing technology. Open source reference alignment tools were optimized to evaluate PCR-amplified STR loci using a custom designed STR genome reference. Evaluation of this approach demonstrated that the 13 CODIS STR loci and amelogenin (AMEL) locus could be accurately called from individual and mixture samples. Sensitivity analysis showed that as few as 18,500 reads, aligned to an in silico referenced genome, were required to genotype an individual (>99% confidence) for the CODIS loci. The power of this technology was further demonstrated by identification of variant alleles containing single nucleotide polymorphisms (SNPs) and the development of quantitative measurements (reads) for resolving mixed samples.
Indexed as
Identifiers
25621315PMC4301848W2321984718What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.