ArticleFrontiers in plant science2014
Garlic γ-glutamyl transpeptidases that catalyze deglutamylation of biosynthetic intermediate of alliin.
Article in Frontiers in plant science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 74 citations in OpenAlex.
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- Integrated volatile metabolomic and transcriptomic analysis provides insights into the regulation of odor components between two Ferula species.BMC plant biology · 2025Article
- Identification of S-1-propenyl-Food chemistry. Molecular sciences · 2025Article
- Development of phytochemical genomics: From decoding metabolome to functional genomics and biotechnology of plant metabolism.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2025Review
- Effect of Temperature Conditions on the Physicochemical Quality of Aged Black Garlic.Foods (Basel, Switzerland) · 2024Article
- Overlooked and misunderstood: can glutathione conjugates be clues to understanding plant glutathione transferases?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Benchmarking Bulb Yield, Medicinal Sulfur Compounds, and Mineral Nutrition of Garlic Varieties.ACS omega · 2024Article
- Genes encoding γ‑glutamyl‑transpeptidases in the allicin biosynthetic pathway in garlic (Biomedical reports · 2024Article
- Understanding the defense mechanism ofFrontiers in plant science · 2024Article
- Effect of Thermal Processes onFoods (Basel, Switzerland) · 2023Article
- Comparative analysis of two kinds of garlic seedings: qualities and transcriptional landscape.BMC genomics · 2023Article
- Targeted Metabolome Profiling of Indonesian Shallots and Japanese Long-Day/Short-Day Bulb Onions.Metabolites · 2022Article
- The ability of callus tissues induced from three Allium plants to accumulate health-beneficial natural products, S-alk(en)ylcysteine sulfoxides.Journal of natural medicines · 2022Article
- Alterations in the Physicochemical Properties and Antioxidant Activity during Aging of Stored Raw Garlic.Foods (Basel, Switzerland) · 2022Article
- Assessment of standardization of domestic commercial black garlic extract forFood science and biotechnology · 2022Article
- Physiological, transcriptomic, and metabolic analyses reveal that mild salinity improves the growth, nutrition, and flavor properties of hydroponic Chinese chive (Frontiers in nutrition · 2022Article
- Antioxidant Properties and Structure-Antioxidant Activity Relationship ofMolecules (Basel, Switzerland) · 2021Review
- Characteristics, biosynthesis, decomposition, metabolism and functions of the garlic odour precursor,Experimental and therapeutic medicine · 2020Review
- Chemistry of aged garlic: Diversity of constituents in aged garlic extract and their production mechanisms via the combination of chemical and enzymatic reactions.Experimental and therapeutic medicine · 2020Review
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8 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
S-Alk(en)yl-L-cysteine sulfoxides are pharmaceutically important secondary metabolites produced by plants that belong to the genus Allium. Biosynthesis of S-alk(en)yl-L-cysteine sulfoxides is initiated by S-alk(en)ylation of glutathione, which is followed by the removal of glycyl and γ-glutamyl groups and S-oxygenation. However, most of the enzymes involved in the biosynthesis of S-alk(en)yl-L-cysteine sulfoxides in Allium plants have not been identified. In this study, we identified three genes, AsGGT1, AsGGT2, and AsGGT3, from garlic (Allium sativum) that encode γ-glutamyl transpeptidases (GGTs) catalyzing the removal of the γ-glutamyl moiety from a putative biosynthetic intermediate of S-allyl-L-cysteine sulfoxide (alliin). The recombinant proteins of AsGGT1, AsGGT2, and AsGGT3 exhibited considerable deglutamylation activity toward a putative alliin biosynthetic intermediate, γ-glutamyl-S-allyl-L-cysteine, whereas these proteins showed very low deglutamylation activity toward another possible alliin biosynthetic intermediate, γ-glutamyl-S-allyl-L-cysteine sulfoxide. The deglutamylation activities of AsGGT1, AsGGT2, and AsGGT3 toward γ-glutamyl-S-allyl-L-cysteine were elevated in the presence of the dipeptide glycylglycine as a γ-glutamyl acceptor substrate, although these proteins can act as hydrolases in the absence of a proper acceptor substrate, except water. The apparent K m values of AsGGT1, AsGGT2, and AsGGT3 for γ-glutamyl-S-allyl-L-cysteine were 86 μM, 1.1 mM, and 9.4 mM, respectively. Subcellular distribution of GFP-fusion proteins transiently expressed in onion cells suggested that AsGGT2 localizes in the vacuole, whereas AsGGT1 and AsGGT3 possess no apparent transit peptide for localization to intracellular organelles. The different kinetic properties and subcellular localizations of AsGGT1, AsGGT2, and AsGGT3 suggest that these three GGTs may contribute differently to the biosynthesis of alliin in garlic.
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