ArticleChembiochem : a European journal of chemical biology2015
Shear-stress-mediated refolding of proteins from aggregates and inclusion bodies.
Article in Chembiochem : a European journal of chemical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 94 citations in OpenAlex.
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- Nanogold Foundry Involving High-Shear-Mediated Photocontact Electrification in Water.Small science · 2024Article
- Vortex fluidic regulated phospholipid equilibria involving liposomes down to sub-micelle size assemblies.Nanoscale advances · 2024Article
- Current state of molecular and metabolic strategies for the improvement of L-asparaginase expression in heterologous systems.Frontiers in pharmacology · 2023Review
- Recombinant expression and biophysical characterization of Mrt4 protein that involved in mRNA turnover and ribosome assembly fromBioengineered · 2022Article
- Vortex Fluidic Mediated Oxidative Sulfitolysis of Oxytocin.Molecules (Basel, Switzerland) · 2022Article
- In Situ Monitored Vortex Fluidic-Mediated Protein Refolding/Unfolding Using an Aggregation-Induced Emission Bioprobe.Molecules (Basel, Switzerland) · 2021Article
- Sub-micron moulding topological mass transport regimes in angled vortex fluidic flow.Nanoscale advances · 2021Article
- Vortex Fluidic-Mediated Fabrication of Fast Gelated Silica Hydrogels with Embedded Laccase Nanoflowers for Real-Time Biosensing under Flow.ACS applied materials & interfaces · 2020Article
- A droplet reactor on a super-hydrophobic surface allows control and characterization of amyloid fibril growth.Communications biology · 2020Article
- Continuous flow vortex fluidic-mediated exfoliation and fragmentation of two-dimensional MXene.Royal Society open science · 2020Article
- A hyper-branched polymer tunes the size and enhances the fluorescent properties of aggregation-induced emission nanoparticles.Nanoscale advances · 2020Article
- Article
- Dynamic thin film mediated slicing of boron nitride nanotubes.Nanoscale advances · 2019Article
- Three-step-in-one synthesis of supercapacitor MWCNT superparamagnetic magnetite composite material under flow.Nanoscale advances · 2019Article
- Neutron imaging and modelling inclined vortex driven thin films.Scientific reports · 2019Article
- Introduction into nanotechnology and microbiology.Methods in microbiology · 2019Article
- Article
- Laser-Ablated Vortex Fluidic-Mediated Synthesis of Superparamagnetic Magnetite Nanoparticles in Water Under Flow.ACS omega · 2018Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
Recombinant protein overexpression of large proteins in bacteria often results in insoluble and misfolded proteins directed to inclusion bodies. We report the application of shear stress in micrometer-wide, thin fluid films to refold boiled hen egg white lysozyme, recombinant hen egg white lysozyme, and recombinant caveolin-1. Furthermore, the approach allowed refolding of a much larger protein, cAMP-dependent protein kinase A (PKA). The reported methods require only minutes, which is more than 100 times faster than conventional overnight dialysis. This rapid refolding technique could significantly shorten times, lower costs, and reduce waste streams associated with protein expression for a wide range of industrial and research applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.