Evidence map›Paper›PMID 25620410›Full record

ReviewCell and tissue research2015

Quantum dots for quantitative imaging: from single molecules to tissue.

Tania Q Vu, Wai Yan Lam, Ellen W Hatch, Diane S Lidke

Abstract readReview
In one paragraph

Review in Cell and tissue research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Review
  7. Article
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  11. EGFR Expression in HER2-Driven Breast Cancer Cells.International journal of molecular sciences · 2020
    Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Nanoscale delivery systems for microRNAs in cancer therapy.Cellular and molecular life sciences : CMLS · 2020
    Review
  17. Article
  18. Article
  19. Optical Probes for Neurobiological Sensing and Imaging.Accounts of chemical research · 2018
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Tania Q VuDepartment of Biomedical Engineering, School of Medicine, Oregon Health and Science University, Portland, Ore., USA, vuta@ohsu.edu.
Wai Yan Lam
Ellen W Hatch
Diane S Lidke

Funding

UNM TRAININGP50GM085273 · NIGMS · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI LIDKE, KEITH A · 2009 to 2018
$26.1M
Ultrastructure and Single Particle Microscopy CoreP30NS061800 · NINDS · OREGON HEALTH & SCIENCE UNIVERSITY · PI AICHER, SUE A · 2009 to 2020
$6.6M
Neuronal signal transduction in space and time using single quantum dotsR01NS071116 · NINDS · OREGON HEALTH & SCIENCE UNIVERSITY · PI VU, TOTHU Q · 2011 to 2015
$1.7M
Single Molecule Imaging to Quantify FcεRI Signaling DynamicsR01GM100114 · NIGMS · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI LIDKE, DIANE · 2012 to 2016
$1.4M
Validation of the use of quantum dot-labeled proteins as biomarkers in vivoR21NS073113 · NINDS · UNIVERSITY OF NEVADA RENO · PI VON BARTHELD, CHRISTOPHER S · 2010 to 2011
$367k
NIGMS NIH HHS 1R01GM100114NIGMS NIH HHS P50 GM085273NIGMS NIH HHS P50GM085273NIGMS NIH HHS R01 GM100114NINDS NIH HHS 1R01NS071116NINDS NIH HHS 1R21NS073113NINDS NIH HHS P30 NS061800NINDS NIH HHS P30-NS061800NINDS NIH HHS R01 NS071116NINDS NIH HHS R21 NS073113
6 · The paper itself

Abstract

Since their introduction to biological imaging, quantum dots (QDs) have progressed from a little known, but attractive, technology to one that has gained broad application in many areas of biology. The versatile properties of these fluorescent nanoparticles have allowed investigators to conduct biological studies with extended spatiotemporal capabilities that were previously not possible. In this review, we focus on QD applications that provide enhanced quantitative information concerning protein dynamics and localization, including single particle tracking and immunohistochemistry, and finish by examining the prospects of upcoming applications, such as correlative light and electron microscopy and super-resolution. Advances in single molecule imaging, including multi-color and three-dimensional QD tracking, have provided new insights into the mechanisms of cell signaling and protein trafficking. New forms of QD tracking in vivo have allowed the observation of biological processes at molecular level resolution in the physiological context of the whole animal. Further methodological development of multiplexed QD-based immunohistochemistry assays should enable more quantitative analysis of key proteins in tissue samples. These advances highlight the unique quantitative data sets that QDs can provide to further our understanding of biological and disease processes.

Indexed as

Organ SpecificityAnimalsCell SurvivalFluorescent DyesImmunohistochemistryMolecular ImagingQuantum DotsFluorescent Dyes

Identifiers

PMID25620410
PMCPMC4382208

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.