ArticlePhysical biology2015
Acute shear stress direction dictates adherent cell remodeling and verifies shear profile of spinning disk assays.
Article in Physical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed.
- Expansion of induced pluripotent stem cells under consideration of bioengineering aspects: part 2.Applied microbiology and biotechnology · 2025Article
- Expansion of induced pluripotent stem cells under consideration of bioengineering aspects: part 1.Applied microbiology and biotechnology · 2025Article
- Advances in cancer mechanobiology: Metastasis, mechanics, and materials.APL bioengineering · 2024Review
- Integrating shear flow and trypsin treatment to assess cell adhesion strength.Biointerphases · 2023Article
- The Double-Edged Sword of Erythrocytes in Health and Disease via Their Adhesiveness.International journal of molecular sciences · 2023Review
- Titrating Avidity of Yeast-Displayed Proteins Using a Transcriptional Regulator.ACS synthetic biology · 2023Article
- Correlating single-molecule rupture mechanics with cell population adhesion by yeast display.Biophysical reports · 2022Article
- Red Blood Cells: Tethering, Vesiculation, and Disease in Micro-Vascular Flow.Diagnostics (Basel, Switzerland) · 2021Article
- Metastatic Esophageal Carcinoma Cells Exhibit Reduced Adhesion Strength and Enhanced Thermogenesis.Cells · 2021Article
- Metastatic State of Cancer Cells May Be Indicated by Adhesion Strength.Biophysical journal · 2017Article
- Biophysical Tools to Study Cellular Mechanotransduction.Bioengineering (Basel, Switzerland) · 2017Review
- Concise Review: Organ Engineering: Design, Technology, and Integration.Stem cells (Dayton, Ohio) · 2017Review
- Mechanical behavior and wall remodeling of blood vessels under axial twist.Yi yong sheng wu li xue · 2016Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Several methods have been developed to quantify population level changes in cell attachment strength given its large heterogeneity. One such method is the rotating disk chamber or 'spinning disk' in which a range of shear forces are applied to attached cells to quantify detachment force, i.e. attachment strength, which can be heterogeneous within cell populations. However, computing the exact force vectors that act upon cells is complicated by complex flow fields and variable cell morphologies. Recent observations suggest that cells may remodel their morphology and align during acute shear exposure, but contrary to intuition, shear is not orthogonal to the radial direction. Here we theoretically derive the magnitude and direction of applied shear and demonstrate that cells, under certain physiological conditions, align in this direction within minutes. Shear force magnitude is also experimentally verified which validates that for spread cells shear forces and not torque or drag dominate in this assay, and demonstrates that the applied force per cell area is largely independent of initial morphology. These findings suggest that direct quantified comparison of the effects of shear on a wide array of cell types and conditions can be made with confidence using this assay without the need for computational or numerical modeling.
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Registered trials
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