ArticleBiochemistry2015
Effect of interior loop length on the thermal stability and pK(a) of i-motif DNA.
Article in Biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 67 citations in OpenAlex.
- Minor groove tetrads: a potent and versatile capping interaction for i-motif structures.Biophysical reviews · 2026Review
- Effects of flanking regions on DNA i-motif folding and stability.Nucleic acids research · 2025Article
- iMab antibody binds single-stranded cytosine-rich sequences and unfolds DNA i-motifs.Nucleic acids research · 2024Article
- Recent Advances in Aptamer-Based Biosensors for Bacterial Detection.Biosensors · 2024Review
- In-cell NMR suggests that DNA i-motif levels are strongly depleted in living human cells.Nature communications · 2024Article
- Emerging roles of i-motif in gene expression and disease treatment.Frontiers in pharmacology · 2023Review
- A single molecule investigation of i-motif stability, folding intermediates, and potential asFrontiers in molecular biosciences · 2022Article
- Engineering DNA quadruplexes in DNA nanostructures for biosensor construction.Nano research · 2022Review
- MD-TSPC4: Computational Method for Predicting the Thermal Stability of I-Motif.International journal of molecular sciences · 2020Article
- A New Design for the Fixed Primer Regions in an Oligonucleotide Library for SELEX Aptamer Screening.Frontiers in chemistry · 2020Article
- Co-Localization of DNA i-Motif-Forming Sequences and 5-Hydroxymethyl-cytosines in Human Embryonic Stem Cells.Molecules (Basel, Switzerland) · 2019Article
- Article
- Systematic investigation of sequence requirements for DNA i-motif formation.Nucleic acids research · 2019Article
- Monitoring of pH Using an i-Motif-Forming Sequence Containing a Fluorescent Cytosine Analogue, tC.Molecules (Basel, Switzerland) · 2019Article
- Article
- Unusual Isothermal Hysteresis in DNA i-Motif pH Transitions: A Study of the RAD17 Promoter Sequence.Biophysical journal · 2018Article
- i-Motif of cytosine-rich human telomere DNA fragments containing natural base lesions.Nucleic acids research · 2018Article
- Probing the Potential Role of Non-B DNA Structures at Yeast Meiosis-Specific DNA Double-Strand Breaks.Biophysical journal · 2017Article
- Identification of multiple genomic DNA sequences which form i-motif structures at neutral pH.Nucleic acids research · 2017Article
- Structure, Properties, and Biological Relevance of the DNA and RNA G-Quadruplexes: Overview 50 Years after Their Discovery.Biochemistry. Biokhimiia · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The four-stranded i-motif (iM) conformation of cytosine-rich DNA is important in a wide variety of biochemical systems ranging from its use in nanomaterials to a potential role in oncogene regulation. An iM is stabilized by acidic pH that allows hemiprotonated cytidines to form a C·C(+) base pair. Fundamental studies that aim to understand how the lengths of loops connecting the protonated C·C(+) pairs affect intramolecular iM physical properties are described here. We characterized both the thermal stability and the pK(a) of intramolecular iMs with differing loop lengths, in both dilute solutions and solutions containing molecular crowding agents. Our results showed that intramolecular iMs with longer central loops form at pHs and temperatures higher than those of iMs with longer outer loops. Our studies also showed that increases in thermal stability of iMs when molecular crowding agents are present are dependent on the loop that is lengthened. However, the increase in pK(a) for iMs when molecular crowding agents are present is insensitive to loop length. Importantly, we also determined the proton activity of solutions containing high concentrations of molecular crowding agents to ascertain whether the increase in pK(a) of an iM is caused by alteration of this activity in buffered solutions. We determined that crowding agents alone increase the apparent pK(a) of a number of small molecules as well as iMs but that increases to iM pK(a) were greater than that expected from a shift in proton activity.
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Registered trials
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