ArticleMolecular and cellular biochemistry2015
Crucial roles of canonical Runx2-dependent pathway on Wnt1-induced osteoblastic differentiation of human periodontal ligament fibroblasts.
Article in Molecular and cellular biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Peripheral blood mesenchymal stem cell-derived exosomes improve renal sympathetic denervation efficacy through β-catenin-mediated cardiac reprogramming.Clinical and translational medicine · 2025Article
- In-vitro evaluation of the effect of okra (Abelmoschus esculentus L.) extract on periodontal cells: a comprehensive study of cellular and molecular impacts.BMC complementary medicine and therapies · 2025Article
- Effects of tetrahedral DNA nanostructures on the treatment of osteoporosis.Cell proliferation · 2024Article
- miR-148a-3p facilitates osteogenic differentiation of fibroblasts in ankylosing spondylitis by activating the Wnt pathway and targeting DKK1.Experimental and therapeutic medicine · 2022Article
- TLE4 Is a Critical Mediator of Osteoblast and Runx2-Dependent Bone Development.Frontiers in cell and developmental biology · 2021Article
- Exendin-4 promotes the osteogenic differentiation of osteoblasts via the Hedgehog/Gli1 signaling pathway.American journal of translational research · 2018Article
- The Interplay of SIRT1 and Wnt Signaling in Vascular Calcification.Frontiers in cardiovascular medicine · 2018Review
- Short-term application of dexamethasone on stem cells derived from human gingiva reduces the expression of RUNX2 and β-catenin.The Journal of international medical research · 2017Article
- Wnt co-receptors Lrp5 and Lrp6 differentially mediate Wnt3a signaling in osteoblasts.PloS one · 2017Article
- Inhibition of Runx2 signaling by TNF-α in ST2 murine bone marrow stromal cells undergoing osteogenic differentiation.In vitro cellular & developmental biology. Animal · 2016Article
- Effects of fluoride on proliferation and mineralization in periodontal ligament cells in vitro.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2016Article
- High insulin levels in KK-Ay diabetic mice cause increased cortical bone mass and impaired trabecular micro-structure.International journal of molecular sciences · 2015Article
- Potential mechanisms underlying the Runx2 induced osteogenesis of bone marrow mesenchymal stem cells.American journal of translational research · 2015Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Canonical Wnt signaling is thought to enhance osteogenic differentiation of human periodontal ligament fibroblasts (hPLFs). However, the mechanism of this enhancement has not yet been defined. We investigated the effects of Wnt1 on osteoblast differentiation of hPLFs and explored the mechanisms of the effects. Treating hPLFs with Wnt1 induced cytosolic accumulation and nuclear translocation of β-catenin with concomitant increases in alkaline phosphatase (ALP) activity and calcium content in a time-dependent and dose-dependent manner. Wnt1-stimulated differentiation of hPLFs was accompanied by augmented phosphorylation of glycogen synthase kinase (GSK)-3β and expression of the bone-specific factors runt-related transcription factor 2 (Runx2), osterix2 (Osx2), ALP, type I collagen, osteopontin, and osteocalcin. Pretreatment with Dickkopf-1 inhibited Wnt1-stimulated differentiation of hPLFs by suppressing GSK-3β phosphorylation, nuclear translocation of β-catenin, and expression of the bone-specific factors. Small interfering (si) RNA-mediated knockdown of β-catenin, or pretreatment with FH535, markedly prevented Wnt1-stimulated differentiation of cells by blocking Runx2 and its downstream factors at the mRNA and protein levels. siRNA-mediated silencing of Runx2 also inhibited Wnt1-stimulated mineralization of cells, accompanied by a reduction in the levels of Osx2 and other early and late bone-formation regulatory factors. However, Wnt1-mediated nuclear translocation of β-catenin and GSK-3β phosphorylation were not inhibited by knockdown of Runx2 or FH535. Collectively, our findings suggested that Wnt1 stimulates osteogenic differentiation and mineralization of hPLFs, mainly by activating the canonical Wnt/β-catenin pathway, in which Runx2 is a key downstream regulator.
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