Evidence map›Paper›PMID 25617825›Full record

ArticleExperimental gerontology2015

Adipose-derived stromal vascular fraction cells isolated from old animals exhibit reduced capacity to support the formation of microvascular networks.

Allison L Aird, Christopher D Nevitt, Katelyn Christian, Stuart K Williams, James B Hoying, Amanda J LeBlanc

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental gerontology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 47 citations in OpenAlex.

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  5. State of the field: cellular and exosomal therapeutic approaches in vascular regeneration.American journal of physiology. Heart and circulatory physiology · 2022
    Review
  6. Article
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  10. Thrombospondin-1 in maladaptive aging responses: a concept whose time has come.American journal of physiology. Cell physiology · 2020
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Allison L AirdCardiovascular Innovation Institute, Jewish Hospital and University of Louisville, Louisville, KY 40202, United States.
Christopher D NevittCardiovascular Innovation Institute, Jewish Hospital and University of Louisville, Louisville, KY 40202, United States; Department of Biochemistry and Molecular Biology, Jewish Hospital and University of Louisville, Louisville, KY 40202, United States.
Katelyn ChristianCardiovascular Innovation Institute, Jewish Hospital and University of Louisville, Louisville, KY 40202, United States.
Stuart K WilliamsCardiovascular Innovation Institute, Jewish Hospital and University of Louisville, Louisville, KY 40202, United States; Department of Physiology and Biophysics, Jewish Hospital and University of Louisville, Louisville, KY 40202, United States.
James B HoyingCardiovascular Innovation Institute, Jewish Hospital and University of Louisville, Louisville, KY 40202, United States; Department of Physiology and Biophysics, Jewish Hospital and University of Louisville, Louisville, KY 40202, United States.
Amanda J LeBlancCardiovascular Innovation Institute, Jewish Hospital and University of Louisville, Louisville, KY 40202, United States; Department of Physiology and Biophysics, Jewish Hospital and University of Louisville, Louisville, KY 40202, United States. Electronic address: amanda.leblanc@louisville.edu.
University of Louisville · US

Funding

Mechanisms of plasticity and repair after SCIP30GM103507 · NIGMS · UNIVERSITY OF LOUISVILLE · PI WHITTEMORE, SCOTT R · 2012 to 2015
$4.2M
NIGMS NIH HHS P30 GM103507
6 · The paper itself

Abstract

unlabelledAdipose-derived regenerative and stem cells, defined collectively as the stromal vascular fraction (SVF), support the formation of neovascular networks at the site of implantation. The effect of advancing age on SVF cell population effectiveness towards stimulated neovascularization was evaluated.

methodsSVF was enzymatically isolated from adipose of young (ySVF, 4 months) or old (oSVF, 24 months) Fisher-344 rats, combined with type I collagen and polymerized. Encapsulated SVF was implanted subcutaneously into young Rag1 mice for two or four weeks. Angiogenic function of age-dependent SVF was also extensively evaluated in vitro using standard assays.

resultsIn vitro studies indicated no difference in angiogenic function between ySVF and oSVF (viability, proliferation, migration, and tube-formation). At two weeks post-implantation, there was no age-related difference in percent apoptosis in explanted constructs. By four weeks post-implantation, oSVF implants displayed 36% less total vessels/mm(2), 43% less perfused vessels/mm(2), and exhibited greater percent apoptosis compared to ySVF (n ≥ 12). Blocking thrombospondin-1 (Thbs-1), a protein found to be highly expressed in oSVF but not ySVF, increased the percent of perfused vascular volume and vessel diameters in oSVF constructs after two weeks compared to oSVF implants treated with control antibody.

conclusionsAdvancing donor age reduces the potential of adipose-derived SVF to derive a mature microcirculation, but does not hinder initial angiogenesis. However, modulation of Thbs-1 may improve this outcome. This data suggests that greater pruning, dysfunctional structural adaptation and/or poor maturation with initiation of blood flow may occur in oSVF.

Indexed as

AgingNeovascularization, PhysiologicAdipose TissueAnimalsBiomarkersCell DifferentiationCell ProliferationFemaleIschemiaMaleMiceMicrovesselsRatsRats, Inbred F344Stromal CellsThrombospondin 1BiomarkersThrombospondin 1AgingAngiogenesisApoptosisInosculationIschemiaMaturationThrombospondin-1Vascular remodeling

Identifiers

PMID25617825
PMCPMC4346434
OpenAlexW2086629327

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.