ArticleSteroids2015
Measurement of estradiol, estrone, and testosterone in postmenopausal human serum by isotope dilution liquid chromatography tandem mass spectrometry without derivatization.
Article in Steroids, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Systemic estradiol levels with low-dose vaginal estrogens.Menopause (New York, N.Y.) · 2020Pooled it
- Mimicking women's endocrine milieu in mice for women's health-related studies.npj women's health · 2025Article
- Ultrasensitive Electrochemical Biosensor for Rapid Screening of Chemicals with Estrogenic Effect.Biosensors · 2024Article
- Correlation of Menopausal Symptoms with Serum Estradiol: A Study in Urban Indian Postmenopausal Women.Journal of obstetrics and gynaecology of India · 2022Article
- Liquid chromatography-tandem mass spectrometry for clinical diagnostics.Nature reviews. Methods primers · 2022Review
- A Sensitive and Specific ESI-Positive LC-MS/MS Method for the Quantitation of Estrogens in Human Serum in Under Three Minutes.Journal of chromatographic science · 2021Article
- Analytical bias of automated immunoassays for six serum steroid hormones assessed by LC-MS/MS.Biochemia medica · 2020Article
- Sex-specific Estrogen Levels and Reference Intervals from Infancy to Late Adulthood Determined by LC-MS/MS.The Journal of clinical endocrinology and metabolism · 2020Article
- Effectiveness of a Global Multidisciplinary Supportive and Educational Intervention in Thermal Resort on Anthropometric and Biological Parameters, and the Disease-Free Survival after Breast Cancer Treatment Completion (PACThe).Journal of oncology · 2020Article
- Simultaneous quantification of estrogens and glucocorticoids in human adipose tissue by liquid-chromatography-tandem mass spectrometry.The Journal of steroid biochemistry and molecular biology · 2019Article
- Current strategies for quantification of estrogens in clinical research.The Journal of steroid biochemistry and molecular biology · 2019Review
- Derivatization enhances analysis of estrogens and their bioactive metabolites in human plasma by liquid chromatography tandem mass spectrometry.Analytica chimica acta · 2019Article
- Sensitive simultaneous quantitation of testosterone and estradiol in serum by LC-MS/MS without derivatization and comparison with the CDC HoSt program.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2017Article
- Development of LC-MS/MS Confirmatory Method for the Determination of Testosterone in Bovine Serum.Journal of veterinary research · 2017Article
- A proposal to improve calibration and outlier detection in high-throughput mass spectrometry.Clinical mass spectrometry (Del Mar, Calif.) · 2016Article
- Article
- The Associations Between Body Mass Index, Smoking, and Alcohol Intake with Ovarian Volume in Midlife Women.Journal of women's health (2002) · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundA high-throughput, sensitive, specific, mass spectrometry-based method for quantitating estrone (E1), estradiol (E2), and testosterone (T) in postmenopausal human serum has been developed for clinical research. The method consumes 100μl human serum for each measurement (triplicates consume 300μl) and does not require derivatization. We adapted a commercially available 96-well plate for sample preparation, extraction, and introduction into the mass spectrometer on a single platform.
methodsSteroid extraction from serum samples and mass spectrometer operational parameters were optimized for analysis of estradiol and subsequently applied to other analytes. In addition to determining the limit of detection (LOD) and limit of quantitation (LOQ) from standard curves, a serum LOQ (sLOQ) was determined by addition of known steroid quantities to serum samples. Mass spectrometric method quantitative data were compared to results using a state-of-the-art ELISA (enzyme-linked immunosorbent assay) using stored serum samples from menopausal women.
resultsThe LOD, LOQ, sLOQ was (0.1pg, 0.3pg, 1pg/ml) for estrone, (0.3pg, 1pg, 3pg/ml) for estradiol, and (0.3pg, 1pg, 30pg/ml) for testosterone, respectively. Mass spectrometry accurately determined concentrations of E2 that could not be quantified by immunochemical methods. E1 concentrations measured by mass spectrometry were in all cases significantly lower than the ELISA measurements, suggesting immunoreactive contaminants in serum may interfere with ELISA. The testosterone measurements broadly agreed with each other in that both techniques could differentiate between low, medium and high serum levels.
conclusionsWe have developed and validated a scalable, sensitive assay for trace quantitation of E1, E2 and T in human serum samples in a single assay using sample preparation method and stable isotope dilution mass spectrometry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.