ArticleAmerican journal of physiology. Heart and circulatory physiology2015
Measurement of shear stress-mediated intracellular calcium dynamics in human dermal lymphatic endothelial cells.
Article in American journal of physiology. Heart and circulatory physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Lymphatics-on-a-chip microphysiological system: engineering lymphatic structure and functionLab on a chip · 2026Review
- Mechanically sensitive HSF1 is a key regulator of left-right symmetry breaking in zebrafish embryos.iScience · 2023Article
- Biomechanical control of lymphatic vessel physiology and functions.Cellular & molecular immunology · 2023Review
- Effect of the snake venom component crotamine on lymphatic endothelial cell responses and lymph transport.Microcirculation (New York, N.Y. : 1994) · 2023Article
- Lymphoedema conditions disrupt endothelial barrier functionJournal of the Royal Society, Interface · 2022Article
- Intracellular calcium dynamics of lymphatic endothelial and muscle cells co-cultured in a Lymphangion-Chip under pulsatile flow.The Analyst · 2022Article
- Modeling of Endothelial Calcium Responses within a Microfluidic Generator of Spatio-Temporal ATP and Shear Stress Signals.Micromachines · 2021Article
- Mechanical forces and metabolic changes cooperate to drive cellular memory and endothelial phenotypes.Current topics in membranes · 2021Review
- Cell Deformation at the Air-Liquid Interface Evokes Intracellular CaFrontiers in physiology · 2021Article
- Lymphatic Vessels and Their Surroundings: How Local Physical Factors Affect Lymph Flow.Biology · 2020Review
- Lymphatic Vessel on a Chip with Capability for Exposure to Cyclic Fluidic Flow.ACS applied bio materials · 2020Article
- Roles of sarcoplasmic reticulum CaScientific reports · 2020Article
- Application of microscale culture technologies for studying lymphatic vessel biology.Microcirculation (New York, N.Y. : 1994) · 2019Review
- Impairment of lymphatic endothelial barrier function by X-ray irradiation.International journal of radiation biology · 2019Article
- Entrainment of Lymphatic Contraction to Oscillatory Flow.Scientific reports · 2019Article
- Lymphatic endothelial cell calcium pulses are sensitive to spatial gradients in wall shear stress.Molecular biology of the cell · 2019Article
- Calcium and electrical dynamics in lymphatic endothelium.The Journal of physiology · 2017Article
- A Novel Computational Model Predicts Key Regulators of Chemokine Gradient Formation in Lymph Nodes and Site-Specific Roles for CCL19 and ACKR4.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- The advection of microparticles, MCF-7 and MDA-MB-231 breast cancer cells in response to very low Reynolds numbers.Biomicrofluidics · 2017Article
- ORAI1 Activates Proliferation of Lymphatic Endothelial Cells in Response to Laminar Flow Through Krüppel-Like Factors 2 and 4.Circulation research · 2017Article
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Authors and funding
6 authors.
Funding
Abstract
The shear stress applied to lymphatic endothelial cells (LEC) by lymph flow changes dramatically under normal conditions as well as in response to disease conditions and immune reactions. In general, LEC are known to regulate the contraction frequency and strength of lymphatic pumping in response to shear stress. Intracellular calcium concentration ([Ca(2+)]i) is an important factor that regulates lymphatic contraction characteristics. In this study, we measured changes in the [Ca(2+)]i under different shear stress levels and determined the source of this calcium signal. Briefly, human dermal LEC were cultured in custom-made microchannels for 3 days before loading with 2 µM fura-2 AM, a ratiometric calcium dye to measure [Ca(2+)]i. Step changes in shear stress resulted in a rapid increase in [Ca(2+)]i followed by a gradual return to the basal level and sometimes below the initial baseline (45.2 ± 2.2 nM). The [Ca(2+)]i reached a peak at 126.2 ± 5.6 nM for 10 dyn/cm(2) stimulus, whereas the peak was only 71.8 ± 5.4 nM for 1 dyn/cm(2) stimulus, indicating that the calcium signal depends on the magnitude of shear stress. Removal of the extracellular calcium from the buffer or pharmocological blockade of calcium release-activated calcium (CRAC) channels significantly reduced the peak [Ca(2+)]i, demonstrating a role of extracellular calcium entry. Inhibition of endoplasmic reticulum (ER) calcium pumps showed the importance of intracellular calcium stores in the initiation of this signal. In conclusion, we demonstrated that the shear-mediated calcium signal is dependent on the magnitude of the shear and involves ER store calcium release and extracellular calcium entry.
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