ArticlePlant physiology2015
Crystal structure and functional characterization of photosystem II-associated carbonic anhydrase CAH3 in Chlamydomonas reinhardtii.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Pyrenoid-based CO2-concentrating mechanisms across diverse lineages: convergent features and their applications for engineering.Plant physiology · 2026Review
- Super-resolution expansion microscopy reveals nanoscale protein domains and CObioRxiv : the preprint server for biology · 2026Article
- Elucidating copper ion interactions with carbonic anhydrase: insights from fluorescence quenching and thermodynamic analysis.Photosynthetica · 2026Article
- Regulatory logic of theFrontiers in plant science · 2026Review
- Nanomaterial-Enabled Enhancements in Thylakoid-Based Biofuel Cells.Nanomaterials (Basel, Switzerland) · 2025Review
- Highly Active Carbonic Anhydrase of the Thylakoid Lumen ofPlants (Basel, Switzerland) · 2024Article
- CAH3 fromCells · 2024Review
- Biochemical characterization of a psychrophilic and halotolerant α-carbonic anhydrase from a deep-sea bacterium,AIMS microbiology · 2023Article
- New horizons for building pyrenoid-based CO2-concentrating mechanisms in plants to improve yields.Plant physiology · 2022Review
- α-CAs from Photosynthetic Organisms.International journal of molecular sciences · 2022Review
- A Rapid Method for Detecting Normal or Modified Plant and Algal Carbonic Anhydrase Activity UsingPlants (Basel, Switzerland) · 2022Article
- Review
- Water oxidation by photosystem II is the primary source of electrons for sustained HProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Pyropia yezoensis genome reveals diverse mechanisms of carbon acquisition in the intertidal environment.Nature communications · 2020Article
- Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research.Frontiers in plant science · 2020Article
- Elucidation and genetic intervention of COJournal of biosciences · 2020Article
- Isolation and Expression Analysis of Three Types of α-Carbonic Anhydrases from the Antarctic Alga Chlamydomonas sp. ICE-L under Different Light Stress Treatments.Molecular biotechnology · 2019Article
- An Update on the Metabolic Roles of Carbonic Anhydrases in the Model Alga Chlamydomonas reinhardtii.Metabolites · 2018Review
- Cloning, Expression Analysis and Enzyme Activity Assays of the α-Carbonic Anhydrase Gene from Chlamydomonas sp. ICE-L.Molecular biotechnology · 2018Article
- Tumor antigen glycosaminoglycan modification regulates antibody-drug conjugate delivery and cytotoxicity.Oncotarget · 2017Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In oxygenic photosynthesis, light energy is stored in the form of chemical energy by converting CO2 and water into carbohydrates. The light-driven oxidation of water that provides the electrons and protons for the subsequent CO2 fixation takes place in photosystem II (PSII). Recent studies show that in higher plants, HCO3 (-) increases PSII activity by acting as a mobile acceptor of the protons produced by PSII. In the green alga Chlamydomonas reinhardtii, a luminal carbonic anhydrase, CrCAH3, was suggested to improve proton removal from PSII, possibly by rapid reformation of HCO3 (-) from CO2. In this study, we investigated the interplay between PSII and CrCAH3 by membrane inlet mass spectrometry and x-ray crystallography. Membrane inlet mass spectrometry measurements showed that CrCAH3 was most active at the slightly acidic pH values prevalent in the thylakoid lumen under illumination. Two crystal structures of CrCAH3 in complex with either acetazolamide or phosphate ions were determined at 2.6- and 2.7-Å resolution, respectively. CrCAH3 is a dimer at pH 4.1 that is stabilized by swapping of the N-terminal arms, a feature not previously observed in α-type carbonic anhydrases. The structure contains a disulfide bond, and redox titration of CrCAH3 function with dithiothreitol suggested a possible redox regulation of the enzyme. The stimulating effect of CrCAH3 and CO2/HCO3 (-) on PSII activity was demonstrated by comparing the flash-induced oxygen evolution pattern of wild-type and CrCAH3-less PSII preparations. We showed that CrCAH3 has unique structural features that allow this enzyme to maximize PSII activity at low pH and CO2 concentration.
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