ArticleThe Plant cell2015
Cytoplasmic nucleation and atypical branching nucleation generate endoplasmic microtubules in Physcomitrella patens.
Article in The Plant cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- tRNA-based polycistronic CRISPR/Cas9 system boosts efficiency of multi-gene deletion in the moss Physcomitrella.Plant methods · 2026Article
- Inhibition of Sterol Biosynthesis Alters Tubulin Association with Detergent-Insoluble Membranes and Affects Microtubule Organization in Pollen Tubes ofPlants (Basel, Switzerland) · 2025Article
- Multifaceted modes of γ-tubulin complex recruitment and microtubule nucleation at mitotic centrosomes.The Journal of cell biology · 2023Article
- Kinesin-4 optimizes microtubule orientations for responsive tip growth guidance in moss.The Journal of cell biology · 2023Article
- Armadillo repeat-containing kinesin represents the versatile plus-end-directed transporter in Physcomitrella.Nature plants · 2023Article
- MOR1/MAP215 acts synergistically with katanin to control cell division and anisotropic cell elongation in Arabidopsis.The Plant cell · 2022Article
- Microtubule-associated proteins promote microtubule generation in the absence of γ-tubulin in human colon cancer cells.The Journal of cell biology · 2021Article
- Kinesin-13 and Kinesin-8 Function during Cell Growth and Division in the MossThe Plant cell · 2020Article
- A versatile microfluidic device for highly inclined thin illumination microscopy in the moss Physcomitrella patens.Scientific reports · 2019Article
- The dual role of the centrosome in organizing the microtubule network in interphase.EMBO reports · 2018Article
- The KCH Kinesin Drives Nuclear Transport and Cytoskeletal Coalescence to Promote Tip Cell Growth inThe Plant cell · 2018Article
- Interplay between Ions, the Cytoskeleton, and Cell Wall Properties during Tip Growth.Plant physiology · 2018Review
- Multiple kinesin-14 family members drive microtubule minus end-directed transport in plant cells.The Journal of cell biology · 2017Article
- Review
- Microtubule encounter-based catastrophe in Arabidopsis cortical microtubule arrays.BMC plant biology · 2016Article
- Clustering of a kinesin-14 motor enables processive retrograde microtubule-based transport in plants.Nature plants · 2015Article
- Estimating cellular parameters through optimization procedures: elementary principles and applications.Frontiers in physiology · 2015Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
The mechanism underlying microtubule (MT) generation in plants has been primarily studied using the cortical MT array, in which fixed-angled branching nucleation and katanin-dependent MT severing predominate. However, little is known about MT generation in the endoplasm. Here, we explored the mechanism of endoplasmic MT generation in protonemal cells of Physcomitrella patens. We developed an assay that utilizes flow cell and oblique illumination fluorescence microscopy, which allowed visualization and quantification of individual MT dynamics. MT severing was infrequently observed, and disruption of katanin did not severely affect MT generation. Branching nucleation was observed, but it showed markedly variable branch angles and was occasionally accompanied by the transport of nucleated MTs. Cytoplasmic nucleation at seemingly random locations was most frequently observed and predominated when depolymerized MTs were regrown. The MT nucleator γ-tubulin was detected at the majority of the nucleation sites, at which a single MT was generated in random directions. When γ-tubulin was knocked down, MT generation was significantly delayed in the regrowth assay. However, nucleation occurred at a normal frequency in steady state, suggesting the presence of a γ-tubulin-independent backup mechanism. Thus, endoplasmic MTs in this cell type are generated in a less ordered manner, showing a broader spectrum of nucleation mechanisms in plants.
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