ArticleApplied and environmental microbiology2015
Large-Scale 13C flux profiling reveals conservation of the Entner-Doudoroff pathway as a glycolytic strategy among marine bacteria that use glucose.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed.
- Metabolic Flux Analysis Reveals Entner-Doudoroff Pathway Dominance in Heterotrophic Deep-Sea Bacterial Isolates.Environmental microbiology reports · 2026Article
- Decoding metabolic trade-offs inSynthetic and systems biotechnology · 2025Article
- Ecogenomics and functional biogeography of the Roseobacter group in the global oceans based on 653 MAGs and SAGs.Microbiome · 2025Article
- Metabolic Redox Coupling Controls Methane Production in Permafrost-Affected Peatlands Through Organic Matter Quality-Dependent Energy Allocation.Global change biology · 2025Article
- Comparative metagenomics indicates metabolic niche differentiation of benthic and planktonic Woeseiaceae.Environmental microbiome · 2025Article
- Vibrio natriegens: Application of a Fast-Growing Halophilic Bacterium.Advances in biochemical engineering/biotechnology · 2025Review
- Glucose priming effect on microbial intercellular metabolic flux diversity in a marine intertidal sediment.PloS one · 2025Article
- Systems biology approach for enhancing limonene yield by re-engineering Escherichia coli.NPJ systems biology and applications · 2024Article
- Genomic Analysis of NovelMarine drugs · 2024Article
- Metagenome-assembled genomes reveal greatly expanded taxonomic and functional diversification of the abundant marine Roseobacter RCA cluster.Microbiome · 2023Article
- Characterization of a bloom-associated alphaproteobacterial lineage, 'Candidatus Phycosocius': insights into freshwater algal-bacterial interactions.ISME communications · 2023Article
- You Exude What You Eat: How Carbon-, Nitrogen-, and Sulfur-Rich Organic Substrates Shape Microbial Community Composition and the Dissolved Organic Matter Pool.Applied and environmental microbiology · 2022Article
- In situ diversity of metabolism and carbon use efficiency among soil bacteria.Science advances · 2022Article
- TCA cycle enhancement and uptake of monomeric substrates support growth of marine Roseobacter at low temperature.Communications biology · 2022Article
- Hot isopropanol quenching procedure for automated microtiter plate scaleMicrobial cell factories · 2022Article
- GC/MS-basedMicrobial cell factories · 2022Article
- Metabolic engineering of Escherichia coli for the production of isobutanol: a review.World journal of microbiology & biotechnology · 2021Review
- Genome-Wide Metabolic Reconstruction of the Synthesis of Polyhydroxyalkanoates from Sugars and Fatty Acids byMicroorganisms · 2021Review
- Restoration of fitness lost due to dysregulation of the pyruvate dehydrogenase complex is triggered by ribosomal binding site modifications.Cell reports · 2021Article
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9 authors.
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Abstract
Marine bacteria form one of the largest living surfaces on Earth, and their metabolic activity is of fundamental importance for global nutrient cycling. Here, we explored the largely unknown intracellular pathways in 25 microbes representing different classes of marine bacteria that use glucose: Alphaproteobacteria, Gammaproteobacteria, and Flavobacteriia of the Bacteriodetes phylum. We used (13)C isotope experiments to infer metabolic fluxes through their carbon core pathways. Notably, 90% of all strains studied use the Entner-Doudoroff (ED) pathway for glucose catabolism, whereas only 10% rely on the Embden-Meyerhof-Parnas (EMP) pathway. This result differed dramatically from the terrestrial model strains studied, which preferentially used the EMP pathway yielding high levels of ATP. Strains using the ED pathway exhibited a more robust resistance against the oxidative stress typically found in this environment. An important feature contributing to the preferential use of the ED pathway in the oceans could therefore be enhanced supply of NADPH through this pathway. The marine bacteria studied did not specifically rely on a distinct anaplerotic route, but the carboxylation of phosphoenolpyruvate (PEP) or pyruvate for fueling of the tricarboxylic acid (TCA) cycle was evenly distributed. The marine isolates studied belong to clades that dominate the uptake of glucose, a major carbon source for bacteria in seawater. Therefore, the ED pathway may play a significant role in the cycling of mono- and polysaccharides by bacterial communities in marine ecosystems.
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