ArticleApplied and environmental microbiology2015
Large fractions of CO2-fixing microorganisms in pristine limestone aquifers appear to be involved in the oxidation of reduced sulfur and nitrogen compounds.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 114 citations in OpenAlex.
- Microbial ecology of shallow groundwater ecosystems.Nature reviews. Microbiology · 2026Review
- Two worlds beneath: Distinct microbial strategies of the rock-attached and planktonic subsurface biosphere.Microbiome · 2026Article
- Diversity and ecological roles of hidden viral players in groundwater microbiomes.Nature communications · 2026Article
- Spatial and Vertical Stratification of Groundwater Microbial Communities Reveals Proteobacterial Dominance and Redox-Driven Ecological Transitions.Microorganisms · 2026Article
- Implications for nitrogen and sulphur cycles: phylogeny and niche-range ofISME communications · 2024Article
- Metabolic and phylogenetic diversity in the phylumISME communications · 2024Article
- Diverse Microbial Hot Spring Mat Communities at Black Canyon of the Colorado River.Microbial ecology · 2023Article
- Differential contribution of nitrifying prokaryotes to groundwater nitrification.The ISME journal · 2023Article
- A glimpse of the paleome in endolithic microbial communities.Microbiome · 2023Article
- Linking Groundwater to Surface Discharge Ecosystems: Archaeal, Bacterial, and Eukaryotic Community Diversity and Structure in Quebec (Canada).Microorganisms · 2023Article
- Microbial degradation and assimilation of veratric acid in oxic and anoxic groundwaters.Frontiers in microbiology · 2023Article
- Seasonal variations of microbial community structure, assembly processes, and influencing factors in karst river.Frontiers in microbiology · 2023Article
- Nitrogen cycling and microbial cooperation in the terrestrial subsurface.The ISME journal · 2022Article
- Bolstering fitness via COThe ISME journal · 2022Article
- Microbial diversity in extreme environments.Nature reviews. Microbiology · 2022Review
- Community characteristics of autotrophic COFrontiers in microbiology · 2022Article
- Microbiome characterization of alpine water springs for human consumption reveals site- and usage-specific microbial signatures.Frontiers in microbiology · 2022Article
- Activity and electron donor preference of two denitrifying bacterial strains identified by Raman gas spectroscopy.Analytical and bioanalytical chemistry · 2022Article
- The microbial dimension of submarine groundwater discharge: current challenges and future directions.FEMS microbiology reviews · 2021Review
- Nitrate Removal by a Novel Lithoautotrophic Nitrate-Reducing, Iron(II)-Oxidizing Culture Enriched from a Pyrite-Rich Limestone Aquifer.Applied and environmental microbiology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The traditional view of the dependency of subsurface environments on surface-derived allochthonous carbon inputs is challenged by increasing evidence for the role of lithoautotrophy in aquifer carbon flow. We linked information on autotrophy (Calvin-Benson-Bassham cycle) with that from total microbial community analysis in groundwater at two superimposed-upper and lower-limestone groundwater reservoirs (aquifers). Quantitative PCR revealed that up to 17% of the microbial population had the genetic potential to fix CO2 via the Calvin cycle, with abundances of cbbM and cbbL genes, encoding RubisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) forms I and II, ranging from 1.14 × 10(3) to 6 × 10(6) genes liter(-1) over a 2-year period. The structure of the active microbial communities based on 16S rRNA transcripts differed between the two aquifers, with a larger fraction of heterotrophic, facultative anaerobic, soil-related groups in the oxygen-deficient upper aquifer. Most identified CO2-assimilating phylogenetic groups appeared to be involved in the oxidation of sulfur or nitrogen compounds and harbored both RubisCO forms I and II, allowing efficient CO2 fixation in environments with strong oxygen and CO2 fluctuations. The genera Sulfuricella and Nitrosomonas were represented by read fractions of up to 78 and 33%, respectively, within the cbbM and cbbL transcript pool and accounted for 5.6 and 3.8% of 16S rRNA sequence reads, respectively, in the lower aquifer. Our results indicate that a large fraction of bacteria in pristine limestone aquifers has the genetic potential for autotrophic CO2 fixation, with energy most likely provided by the oxidation of reduced sulfur and nitrogen compounds.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.