Evidence map›Paper›PMID 25616398›Full record

ArticleReproductive sciences (Thousand Oaks, Calif.)2015

Chorioamnionitis Occurring in Women With Preterm Rupture of the Fetal Membranes Is Associated With a Dynamic Increase in mRNAs Coding Cytokines in the Maternal Circulation.

Owen Stock, Lavinia Gordon, Jada Kapoor, Susan P Walker, Clare Whitehead, Tu'uhevaha J Kaitu'u-Lino, Gabrielle Pell, Natalie J Hannan, Stephen Tong

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive sciences (Thousand Oaks, Calif.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 22 citations in OpenAlex.

  1. Review
  2. Article
  3. Blood gene transcript signature profiling in pregnancies resulting in preterm birth: A systematic review.European journal of obstetrics & gynecology and reproductive biology: X · 2020
    Review
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Owen StockDepartment of Obstetrics and Gynaecology, Translational Obstetrics Group, University of Melbourne, Mercy Hospital for Women, Heidelberg, Victoria, Australia owen.stock@unimelb.edu.au.
Lavinia GordonMurdoch Children's Research Institute, The Royal Children's Hospital, Parkville, Victoria, Australia Australian Genome Research Facility Ltd, The Walter and Eliza Hall Institute, Parkville, Victoria, Australia.
Jada KapoorDepartment of Obstetrics and Gynaecology, Translational Obstetrics Group, University of Melbourne, Mercy Hospital for Women, Heidelberg, Victoria, Australia.
Susan P WalkerDepartment of Obstetrics and Gynaecology, Translational Obstetrics Group, University of Melbourne, Mercy Hospital for Women, Heidelberg, Victoria, Australia.
Clare WhiteheadDepartment of Obstetrics and Gynaecology, Translational Obstetrics Group, University of Melbourne, Mercy Hospital for Women, Heidelberg, Victoria, Australia.
Tu'uhevaha J Kaitu'u-LinoDepartment of Obstetrics and Gynaecology, Translational Obstetrics Group, University of Melbourne, Mercy Hospital for Women, Heidelberg, Victoria, Australia.
Gabrielle PellDepartment of Obstetrics and Gynaecology, Translational Obstetrics Group, University of Melbourne, Mercy Hospital for Women, Heidelberg, Victoria, Australia.
Natalie J HannanDepartment of Obstetrics and Gynaecology, Translational Obstetrics Group, University of Melbourne, Mercy Hospital for Women, Heidelberg, Victoria, Australia.
Stephen TongDepartment of Obstetrics and Gynaecology, Translational Obstetrics Group, University of Melbourne, Mercy Hospital for Women, Heidelberg, Victoria, Australia.
Mercy Hospital for Women · AURoyal Children's Hospital · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPreterm prelabor rupture of the fetal membranes (PPROM) is a significant contributor to the morbidity and mortality of preterm birth, particularly in the setting of chorioamnionitis. No sensitive or specific diagnostic or predictive test currently exists for the accurate diagnosis of chorioamnionitis. Our aim was to measure messenger RNA (mRNA) coding cytokines in the maternal blood and examine whether they were increased in association with chorioamnionitis at delivery. METHODS/

resultsWe performed a prospective cohort study of women recruited with PPROM at a mean gestational age of 28.9 weeks at risk of developing chorioamnionitis. Blood was sampled from participants, and the expression of mRNA coding for proinflammatory genes was measured in women with and without chorioamnionitis at the time of delivery as well as gestation-matched healthy controls. Expression was measured using quantitative polymerase chain reaction (PCR) and also digital PCR. Interleukin 1β (IL1B) mRNA expression in maternal blood was elevated in women with chorioamnionitis compared to gestation-matched controls. Importantly, among women admitted with PPROM, digital PCR confirmed a significant increase in IL1B expression in maternal blood in women with chorioamnionitis compared to women without chorioamnionitis. Polymerase chain reaction array revealed that CD14, nuclear factor of κ light polypeptide gene enhancer in B-cells 1 (NFKB1), and tumor necrosis factor receptor super family-interacting serine-threonine kinase 1 mRNA were significantly increased in women with chorioamnionitis compared to controls. Digital PCR confirmed that NFKB1 mRNA was significantly increased in patients with chorioamnionitis compared to controls and that CD14 levels increased over time in patients with PPROM having chorioamnionitis.

conclusionMeasuring circulating proinflammatory mRNA in women with PPROM may distinguish those with chorioamnionitis from those without, in turn providing better targeted therapies and appropriate timing of delivery.

Indexed as

AdultBiomarkersChorioamnionitisCohort StudiesCytokinesFemaleHumansInterleukin-1betaLipopolysaccharide ReceptorsNF-kappa B p50 SubunitPlacental CirculationPregnancyPremature Rupture of Fetal MembranesProspective StudiesRNA, MessengerTumor Necrosis Factor-alphaBiomarkersCytokinesIL1B protein, humanInterleukin-1betaLipopolysaccharide ReceptorsNF-kappa B p50 SubunitNFKB1 protein, humanRNA, MessengerTumor Necrosis Factor-alphachorioamnionitisdigital PCRIL1Binflammationintrauterine infectionmRNAPCRpreterm birthpreterm prelabor rupture of the fetal membranes

Identifiers

PMID25616398
PMCPMC4565477
OpenAlexW2084203438

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.