ArticleThe ISME journal2015
Quorum sensing-regulated chitin metabolism provides grazing resistance to Vibrio cholerae biofilms.
Article in The ISME journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 74 citations in OpenAlex.
- Vibrio cholerae-synthesized tryptophan activates innate immune signaling in enteroendocrine cells via phosphorylation of the nuclear receptor ultraspiracle.Nature communications · 2026Article
- Environmental regulation of virulence in Vibrio cholerae: integrating multi-omics, predictive models, and comparative insights across emerging Vibrio species.Gut pathogens · 2026Review
- Rational assembly of synthetic marine biofilm community with chitinase production.Research square · 2026Article
- Additional Binding Domain of Marine Chitinase Increases Binding Specificity and Prolongs the Lifetime of Productive Binding State.Biochemistry · 2026Article
- A conserved, immune-regulated peritrophin promotes Vibrio cholerae colonization of the arthropod intestine.Nature communications · 2026Article
- Article
- Review of the Presence and Phage-Mediated Transfer of ARGs in Biofilms.Microorganisms · 2025Review
- Single-cell RNA-seq reveals a key role forFrontiers in microbiology · 2025Article
- Quorum regulated latent environmental cells of toxigenic Vibrio cholerae and their role in cholera outbreaks.Gut pathogens · 2024Review
- Discovery of Vibrio cholerae in Urban Sewage in Copenhagen, Denmark.Microbial ecology · 2024Article
- Associational Resistance to Predation by Protists in a Mixed Species Biofilm.Applied and environmental microbiology · 2023Article
- New Insights into Vibrio cholerae Biofilms from Molecular Biophysics to Microbial Ecology.Advances in experimental medicine and biology · 2023Article
- Environmental Reservoirs of Pathogenic Vibrio spp. and Their Role in Disease: The List Keeps Expanding.Advances in experimental medicine and biology · 2023Review
- Stress Responses in Pathogenic Vibrios and Their Role in Host and Environmental Survival.Advances in experimental medicine and biology · 2023Article
- Zooplankton act as cruise ships promoting the survival and pathogenicity of pathogenic bacteria.Microbiology and immunology · 2022Review
- Loss of the Acetate Switch in Vibrio vulnificus Enhances Predation Defense against Tetrahymena pyriformis.Applied and environmental microbiology · 2022Article
- Strain-level functional variation in the human gut microbiota based on bacterial binding to artificial food particles.Cell host & microbe · 2021Article
- Chitin Degradation Machinery and Secondary Metabolite Profiles in the Marine BacteriumMarine drugs · 2021Article
- Comparison of chitin-induced natural transformation in pandemic Vibrio cholerae O1 El Tor strains.Environmental microbiology · 2020Article
- Species-Specific Quorum Sensing Represses the Chitobiose Utilization Locus in Vibrio cholerae.Applied and environmental microbiology · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Association of Vibrio cholerae with chitinous surfaces of zooplankton is important for its persistence in marine environments, as it provides accessibility to nutrients and resistance to stresses. Predation by heterotrophic protists has a major impact on the survival of V. cholerae. V. cholerae forms biofilms as its main defensive strategy, and quorum sensing (QS) additionally regulates the production of antiprotozoal factors. The role of chitin and QS regulation in V. cholerae grazing resistance was investigated by exposing V. cholerae wild-type (WT) and QS mutant biofilms grown on chitin flakes to the bacteriotrophic, surface-feeding flagellate Rhynchomonas nasuta. V. cholerae formed more biofilm biomass on chitin flakes compared with nonchitinous surfaces. The growth of R. nasuta was inhibited by WT biofilms grown on chitin flakes, whereas the inhibition was attenuated in QS mutant biofilms. The chitin-dependent toxicity was also observed when the V. cholerae biofilms were developed under continuous flow or grown on a natural chitin source, the exoskeleton of Artemia. In addition, the antiprotozoal activity and ammonium concentration of V. cholerae biofilm supernatants were quantified. The ammonium levels (3.5 mM) detected in the supernatants of V. cholerae WT biofilms grown on chitin flakes were estimated to reduce the number of R. nasuta by >80% in add-back experiments, and the supernatant of QS mutant biofilms was less toxic owing to a decrease in ammonium production. Transcriptomic analysis revealed that the majority of genes involved in chitin metabolism and chemotaxis were significantly downregulated in QS mutant biofilms when grown on chitin compared with the WT biofilms.
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