ArticleThe Journal of biological chemistry2015
Identification and characterization of a novel prokaryotic peptide: N-glycosidase from Elizabethkingia meningoseptica.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- Impacts of N-glycanase1 (NGLY1) Down Regulation on the Function of Mitochondria.Journal of cellular biochemistry · 2026Article
- Article
- Downregulation of Endo-Beta-N-Acetylglucosaminidase in <italic>Caenorhabditis elegans</italic> Improves Stress Adaptivity.Cells, tissues, organs · 2026Article
- Deglycosylation at War: Host N-Glycoprotein Remodeling in Infection and Immunity.Infection and drug resistance · 2026Review
- Structural characterization of zebrafish Ngly2, an ovary-enriched acid PNGase required for egg-free glycan production.The Journal of biological chemistry · 2025Article
- PNGaseL fromRoyal Society open science · 2025Article
- Article
- Functional prediction of the potential NGLY1 mutations associated with rare disease CDG.Heliyon · 2024Article
- Do I Need to Trypsin Digest Before Releasing IgG Glycans With PNGase-F?Journal of biomolecular techniques : JBT · 2023Article
- Enzymatic Investigation ofProteomes · 2023Article
- PlantProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Core-Proteomics-Based Annotation of Antigenic Targets and Reverse-Vaccinology-Assisted Design of Ensemble Immunogen against the Emerging Nosocomial Infection-Causing BacteriumInternational journal of environmental research and public health · 2021Article
- N-Linked Glycosylation on Anthrax Toxin Receptor 1 Is Essential for Seneca Valley Virus Infection.Viruses · 2021Article
- Rapid capillary gel electrophoresis analysis of human milk oligosaccharides for food additive manufacturing in-process control.Analytical and bioanalytical chemistry · 2021Article
- Mass Spectrometry-Based Methods for Immunoglobulin G N-Glycosylation Analysis.Experientia supplementum (2012) · 2021Article
- Article
- Preparation of Complex Glycans From Natural Sources for Functional Study.Frontiers in chemistry · 2020Review
- Filamentation initiated by Cas2 and its association with the acquisition process in cells.International journal of oral science · 2019Article
- Method comparison for N-glycan profiling: Towards the standardization of glycoanalytical technologies for cell line analysis.PloS one · 2019Article
- Identification and characterization of a core fucosidase from the bacteriumThe Journal of biological chemistry · 2018Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Peptide:N-glycosidase (PNGase) F, the first PNGase identified in prokaryotic cells, catalyzes the removal of intact asparagine-linked oligosaccharide chains from glycoproteins and/or glycopeptides. Since its discovery in 1984, PNGase F has remained as the sole prokaryotic PNGase. Recently, a novel gene encoding a protein with a predicted PNGase domain was identified from a clinical isolate of Elizabethkingia meningoseptica. In this study, the candidate protein was expressed in vitro and was subjected to biochemical and structural analyses. The results revealed that it possesses PNGase activity and has substrate specificity different from that of PNGase F. The crystal structure of the protein was determined at 1.9 Å resolution. Structural comparison with PNGase F revealed a relatively larger glycan-binding groove in the catalytic domain and an additional bowl-like domain with unknown function at the N terminus of the candidate protein. These structural and functional analyses indicated that the candidate protein is a novel prokaryotic N-glycosidase. The protein has been named PNGase F-II.
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