ArticlePloS one2015
Novel exons and splice variants in the human antibody heavy chain identified by single cell and single molecule sequencing.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed.
- Combining long-read DNA and RNA sequencing to enhance molecular understanding of structural variations leading to copy gains.Computational and structural biotechnology journal · 2025Article
- LIQA: long-read isoform quantification and analysis.Genome biology · 2021Article
- Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA.Communications biology · 2021Article
- Beyond bulk single-chain sequencing: Getting at the whole receptor.Current opinion in systems biology · 2020Review
- Systematic Profiling of Full-Length Ig and TCR Repertoire Diversity in Rhesus Macaque through Long Read Transcriptome Sequencing.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- TAP: a targeted clinical genomics pipeline for detecting transcript variants using RNA-seq data.BMC medical genomics · 2018Article
- Next-Generation Sequencing of Antibody Display Repertoires.Frontiers in immunology · 2018Review
- Nanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells.Nature communications · 2017Article
- The analysis of clonal expansions in normal and autoimmune B cell repertoires.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Antibody heavy chains contain a variable and a constant region. The constant region of the antibody heavy chain is encoded by multiple groups of exons which define the isotype and therefore many functional characteristics of the antibody. We performed both single B cell RNAseq and long read single molecule sequencing of antibody heavy chain transcripts and were able to identify novel exons for IGHA1 and IGHA2 as well as novel isoforms for IGHM antibody heavy chain.
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Registered trials
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