ArticleJournal of microscopy2015
Array tomography: characterizing FAC-sorted populations of zebrafish immune cells by their 3D ultrastructure.
Article in Journal of microscopy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- Morphomics via next-generation electron microscopy.Journal of molecular cell biology · 2024Review
- Spatial Transcriptomics-correlated Electron Microscopy maps transcriptional and ultrastructural responses to brain injury.Nature communications · 2023Article
- One for All, All for One: A Close Look at In-Resin Fluorescence Protocols for CLEM.Frontiers in cell and developmental biology · 2022Review
- Symplasmic isolation marks cell fate changes during somatic embryogenesis.Journal of experimental botany · 2020Article
- Methods for array tomography with correlative light and electron microscopy.Medical molecular morphology · 2019Review
- Q&A: Array tomography.BMC biology · 2018Article
- Comparison of 3D cellular imaging techniques based on scanned electron probes: Serial block face SEM vs. Axial bright-field STEM tomography.Journal of structural biology · 2018Article
- Hierarchical imaging: a new concept for targeted imaging of large volumes from cells to tissues.BMC cell biology · 2016Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
For 3D reconstructions of whole immune cells from zebrafish, isolated from adult animals by FAC-sorting we employed array tomography on hundreds of serial sections deposited on silicon wafers. Image stacks were either recorded manually or automatically with the newly released ZEISS Atlas 5 Array Tomography platform on a Zeiss FEGSEM. To characterize different populations of immune cells, organelle inventories were created by segmenting individual cells. In addition, arrays were used for quantification of cell populations with respect to the various cell types they contained. The detection of immunological synapses in cocultures of cell populations from thymus or WKM with cancer cells helped to identify the cytotoxic nature of these cells. Our results demonstrate the practicality and benefit of AT for high-throughput ultrastructural imaging of substantial volumes.
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Registered trials
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