ArticleProtein science : a publication of the Protein Society2015
Hydrogen exchange of disordered proteins in Escherichia coli.
Article in Protein science : a publication of the Protein Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Exploring Intrinsic Disorder in Human Synucleins and Associated Proteins.International journal of molecular sciences · 2024Article
- The effects of biological crowders on fibrillization, structure, diffusion, and conformational dynamics of α-synuclein.Protein science : a publication of the Protein Society · 2024Article
- Experimental methods to study the structure and dynamics of intrinsically disordered regions in proteins.Current research in structural biology · 2024Review
- Metal interactions of α-synuclein probed by NMR amide-proton exchange.Frontiers in chemistry · 2023Article
- Ion influence on surface water dynamics and proton exchange at protein surfaces - A unified model for transverse and longitudinal NMR relaxation dispersion.Journal of molecular liquids · 2022Article
- Radio Signals from Live Cells: The Coming of Age of In-Cell Solution NMR.Chemical reviews · 2022Review
- Reduced efficacy of a Src kinase inhibitor in crowded protein solution.Nature communications · 2021Article
- Ensemble description of the intrinsically disordered N-terminal domain of the Nipah virus P/V protein from combined NMR and SAXS.Scientific reports · 2020Article
- Protein Structure Determination in Living Cells.International journal of molecular sciences · 2019Review
- In-Cell NMR Spectroscopy of Intrinsically Disordered Proteins.Proteomics · 2019Review
- Intrinsically disordered proteins in crowded milieu: when chaos prevails within the cellular gumbo.Cellular and molecular life sciences : CMLS · 2018Review
- To be disordered or not to be disordered: is that still a question for proteins in the cell?Cellular and molecular life sciences : CMLS · 2017Review
- Characterization of hydrogen bonding motifs in proteins: hydrogen elimination monitoring by ultraviolet photodissociation mass spectrometry.Physical chemistry chemical physics : PCCP · 2017Article
- Tardigrades Use Intrinsically Disordered Proteins to Survive Desiccation.Molecular cell · 2017Article
- In-cell NMR: a topical review.IUCrJ · 2017Review
- Molecular Effects of Concentrated Solutes on Protein Hydration, Dynamics, and Electrostatics.Biophysical journal · 2016Article
- Protein Composition Determines the Effect of Crowding on the Properties of Disordered Proteins.Biophysical journal · 2016Article
- A Unique Tool for Cellular Structural Biology: In-cell NMR.The Journal of biological chemistry · 2016Review
- In-cell thermodynamics and a new role for protein surfaces.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Intracellular pH modulates quinary structure.Protein science : a publication of the Protein Society · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A truly disordered protein lacks a stable fold and its backbone amide protons exchange with solvent at rates predicted from studies of unstructured peptides. We have measured the exchange rates of two model disordered proteins, FlgM and α-synuclein, in buffer and in Escherichia coli using the NMR experiment, SOLEXSY. The rates are similar in buffer and cells and are close to the rates predicted from data on small, unstructured peptides. This result indicates that true disorder can persist inside the crowded cellular interior and that weak interactions between proteins and macromolecules in cells do not necessarily affect intrinsic rates of exchange.
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