ArticleFrontiers in microbiology2014
Characterization of the genetic environment of bla ESBL genes, integrons and toxin-antitoxin systems identified on large transferrable plasmids in multi-drug resistant Escherichia coli.
Article in Frontiers in microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
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- Metagenomics insights into bacterial diversity and antibiotic resistome of the sewage in the city of Belém, Pará, Brazil.Frontiers in microbiology · 2024Article
- Characterization of integrons, extended spectrum beta lactamases and genetic diversity among uropathogenicIranian journal of microbiology · 2023Article
- Roles of Proteins Containing Immunoglobulin-Like Domains in the Conjugation of Bacterial Plasmids.mSphere · 2022Article
- High Prevalence of Drug Resistance and Class 1 Integrons inFrontiers in microbiology · 2021Article
- Review
- Expression of a novel class of bacterial Ig-like proteins is required for IncHI plasmid conjugation.PLoS genetics · 2019Article
- Plasmid profiling and incompatibility grouping of multidrug resistant Salmonella enterica serovar Typhi isolates in Nairobi, Kenya.BMC research notes · 2019Article
- Emergence and molecular characterization of multidrug-resistantInfection and drug resistance · 2019Article
- Prevalence of Integrons and Insertion Sequences in ESBL-ProducingInternational journal of environmental research and public health · 2018Article
- Genome analyses ofGut pathogens · 2018Article
- Genetic Environment ofFrontiers in microbiology · 2018Article
- Characterization of plasmids harboring blaCTX-M and blaCMY genes in E. coli from French broilers.PloS one · 2018Article
- Understanding resistance.Intensive care medicine · 2016Review
- Toxin-Antitoxin Systems in Clinical Pathogens.Toxins · 2016Review
- IncHI2 Plasmids Are Predominant in Antibiotic-ResistantFrontiers in microbiology · 2016Article
- Whole Genome Sequencing for Genomics-Guided Investigations of Escherichia coli O157:H7 Outbreaks.Frontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesPreviously 14 conjugative plasmids from multi-drug resistant (MDR) Escherichia coli from healthy humans and food-producing animals in Switzerland were sequenced. The aim of this study was to extend the genetic characterization of these plasmids with a focus on bla ESBL genes including bla CTX-M-1 and bla TEM, class 1 integrons and toxin-antitoxin (TA) systems contained therein.
methodsThe nucleotide sequences and subsequent annotation therein of 14 conjugative plasmids were previously determined from their corresponding transconjugants. The TA loci were confirmed by RASTA-Bacteria.
resultsEight of the conjugative plasmids identified were found to encode genes expressing ESBLs. Structural heterogeneity was noted in the regions flanking both the bla CTX-M-1 and bla TEM genes. The bla CTX-M-1 genes were associated with the common insertion sequences ISEcp1 and IS26, and uniquely with an IS5 element in one case; while bla TEM genes were found to be associated with IS26 and Tn2. A new bla TEM-210 gene was identified. Seven class 1 integrons were also identified and assigned into 3 groups, denoted as In54, In369 and In501. Sixteen TA loci belonging to 4 of the TA gene families (relBE, vapBC, ccd and mazEF) were identified on 11 of these plasmids.
conclusionsComparative sequence analysis of these plasmids provided data on the structures likely to contribute to sequence diversity associated with these accessory genes, including IS26, ISEcp1 and Tn2. All of them contribute to the dissemination of the corresponding resistance genes located on the different plasmids. There appears to be no association between β-lactam encoding genes and TA systems.
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