ArticleInternational journal of nanomedicine2015
Barium titanate nanoparticles and hypergravity stimulation improve differentiation of mesenchymal stem cells into osteoblasts.
Article in International journal of nanomedicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Three-Dimensional Printing Methods for Bioceramic-Based Scaffold Fabrication for Craniomaxillofacial Bone Tissue Engineering.Journal of functional biomaterials · 2024Review
- Increased H3K9me3 and F-Actin Reorganization in the Rapid Adaptive Response to Hypergravity in Human T Lymphocytes.International journal of molecular sciences · 2023Article
- Bio-Piezoelectric Ceramic Composites for Electroactive Implants-Biological Performance.Biomimetics (Basel, Switzerland) · 2023Article
- Hypergravity enhances RBM4 expression in human bone marrow-derived mesenchymal stem cells and accelerates their differentiation into neurons.Regenerative therapy · 2023Article
- Transcriptional Response in Human Jurkat T Lymphocytes to a near Physiological Hypergravity Environment and to One Common in Routine Cell Culture Protocols.International journal of molecular sciences · 2023Article
- Artificially altered gravity elicits cell homeostasis imbalance in planarian worms, and cerium oxide nanoparticles counteract this effect.Journal of biomedical materials research. Part A · 2021Article
- Machine Learning Assisted Classification of Cell Lines and Cell States on Quantitative Phase Images.Cells · 2021Article
- Amine modification of calcium phosphate by low-pressure plasma for bone regeneration.Scientific reports · 2021Article
- Modifications of Plasma Membrane Organization in Cancer Cells for Targeted Therapy.Molecules (Basel, Switzerland) · 2021Review
- Self-assembled biomimetic Nano-Matrix for stem cell anchorage.Journal of biomedical materials research. Part A · 2020Article
- Antibody-Conjugated Barium Titanate Nanoparticles for Cell-Specific Targeting.ACS applied nano materials · 2020Article
- Nanostructured Biomaterials for Bone Regeneration.Frontiers in bioengineering and biotechnology · 2020Review
- Fracture Non-Union: A Review of Clinical Challenges and Future Research Needs.Malaysian orthopaedic journal · 2019Review
- The application of nanogenerators and piezoelectricity in osteogenesis.Science and technology of advanced materials · 2019Review
- Release of VEGF from Dental Implant Improves Osteogenetic Process: Preliminary In Vitro Tests.Materials (Basel, Switzerland) · 2017Article
- Physico-electrochemical Characterization of Pluripotent Stem Cells during Self-Renewal or Differentiation by a Multi-modal Monitoring System.Stem cell reports · 2017Article
- Electroactive BaTiOInternational journal of nanomedicine · 2017Article
- Nanoporosity improved water absorption, in vitro degradability, mineralization, osteoblast responses and drug release of poly(butylene succinate)-based composite scaffolds containing nanoporous magnesium silicate compared with magnesium silicate.International journal of nanomedicine · 2017Article
- Effects of hypergravity on the angiogenic potential of endothelial cells.Journal of the Royal Society, Interface · 2016Article
- BMP9-induced osteogenic differentiation is partially inhibited by miR-30a in the mesenchymal stem cell line C3H10T1/2.Journal of molecular histology · 2015Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEnhancement of the osteogenic potential of mesenchymal stem cells (MSCs) is highly desirable in the field of bone regeneration. This paper proposes a new approach for the improvement of osteogenesis combining hypergravity with osteoinductive nanoparticles (NPs). MATERIALS AND
methodsIn this study, we aimed to investigate the combined effects of hypergravity and barium titanate NPs (BTNPs) on the osteogenic differentiation of rat MSCs, and the hypergravity effects on NP internalization. To obtain the hypergravity condition, we used a large-diameter centrifuge in the presence of a BTNP-doped culture medium. We analyzed cell morphology and NP internalization with immunofluorescent staining and coherent anti-Stokes Raman scattering, respectively. Moreover, cell differentiation was evaluated both at the gene level with quantitative real-time reverse-transcription polymerase chain reaction and at the protein level with Western blotting.
resultsFollowing a 20 g treatment, we found alterations in cytoskeleton conformation, cellular shape and morphology, as well as a significant increment of expression of osteoblastic markers both at the gene and protein levels, jointly pointing to a substantial increment of NP uptake. Taken together, our findings suggest a synergistic effect of hypergravity and BTNPs in the enhancement of the osteogenic differentiation of MSCs.
conclusionThe obtained results could become useful in the design of new approaches in bone-tissue engineering, as well as for in vitro drug-delivery strategies where an increment of nanocarrier internalization could result in a higher drug uptake by cell and/or tissue constructs.
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